食品科学 ›› 2010, Vol. 31 ›› Issue (17): 197-200.doi: 10.7506/spkx1002-6630-201017045

• 生物工程 • 上一篇    下一篇

鸭肠道内产蛋白酶乳杆菌的分离筛选

童 敏,潘道东*   

  1. 宁波大学生命科学与生物工程学院
  • 收稿日期:2010-01-11 出版日期:2010-09-15 发布日期:2010-12-29
  • 通讯作者: 潘道东 E-mail:daodongpan@163.com
  • 基金资助:

    国家自然科学基金项目(30972130);江苏省科技支撑计划项目(BE2009366);
    国家农业转化基金项目(2009GB2C220412);国家“863”计划项目(2007AA10Z357)

Isolation and Screening of Protease-producing Lactobacilli in Duck Gastrointestinal Tract

TONG Min,PAN Dao-dong   

  1. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
  • Received:2010-01-11 Online:2010-09-15 Published:2010-12-29
  • Contact: PAN Dao-dong E-mail:daodongpan@163.com

摘要:

分离筛选鸭肠道内产蛋白酶乳杆菌。利用脱脂乳培养基,从鸭消化道中分离筛选出两株产蛋白酶的乳酸菌,对菌体形态、染色反应、培养性状、生理生化性状进行系统研究,结合16S rRNA 序列分析鉴定出筛选的乳酸菌为干酪乳杆菌鼠李糖亚种和干酪乳杆菌干酪亚种。

关键词: 乳杆菌, 产蛋白酶, 分离, 鉴定

Abstract:

Two lactic acid bacterial stains having the ability to produce protease were isolated and screened from the gastrointestinal tract of duck with the aid of skim milk medium. The stains were identified as Lactobacillus casei subsp., Rhamnosus and Lactobacillus casei through the systematic investigations of morphology, staining reaction, physiological and biochemical characteristics and 16S rRNA sequences analysis.

Key words: Lactobacillus, producing protease, screening, identification

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