关键词: 大花金挖耳, 悬浮培养, 生物合成, 黄酮类化合物

Abstract:

The goal of the present study was to optimize the culture conditions for the establishment of suspension cell lines from Carpesium macrocephalum callus. The effects of medium type, concentrations of sucrose, VB1, NAA and 6-BA, initial pH value, inoculum quantity and culture time on cell biomass and flavonoid production were examined. The optimal conditions for culture in NT liquid medium conducive to both cell growth and flavonoid production were as follows: initial pH, 5.5, inoculum quantity, 40 g/L; NAA concentration, 1.0 mg/L; and 6-BA concentration, 0.2 mg/L. Cell browning was inhibited by adding 1.0－4.0 mg/L of VB1. Both the growth of Carpesium macrocephalum cells and the synthesis of flavonoids trended to first ascend and then descent with prolonged culture time. A cell biomass ranging from 23.54 to 24.51 g/L was achieved at 15－20 d post-inoculation, and the yield of flavonoids was between 1.19% and 1.23%.

Key words: Carpesium macrocephalum, cell suspension culture, biosynthesis, flavonoid