食品科学 ›› 2010, Vol. 31 ›› Issue (21): 293-297.doi: 10.7506/spkx1002-6630-201021066

• 生物工程 • 上一篇    下一篇

天然发酵茯砖茶微生物菌群及抗菌特性研究

张 浩1,2,李 华3,*,莫海珍2   

  1. 1.西北农林科技大学食品科学与工程学院 2.河南科技学院食品学院 3.西北农林科技大学葡萄酒学院
  • 收稿日期:2010-06-24 修回日期:2010-10-25 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 李华 E-mail:lihuawine@nwsuaf.edu.cn
  • 基金资助:

    河南省高校青年骨干教师资助项目(教高2008 - 755 号);河南省重点科技攻关项目(102102310328)

Microbial Population and Antibacterial Activity in Fuzhuan Brick Tea

ZHANG Hao1,2,LI Hua3,*,MO Hai-zhen2   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
    2. School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China;
    3. College of Enology, Northwest A&F University, Yangling 712100, China
  • Received:2010-06-24 Revised:2010-10-25 Online:2010-11-15 Published:2010-12-29
  • Contact: LI Hua3 E-mail:lihuawine@nwsuaf.edu.cn

摘要:

对天然发酵茯砖茶的微生物菌群以及抗菌活性进行研究。微生物分离和鉴定结果表明:曲霉、青霉和散囊菌是天然发酵茯砖茶的主要微生物,其中散囊菌是真菌发酵过程中的优势菌种。发酵茶水提物的抑菌实验显示,茯砖茶的浸提物对一些食品致病菌有抑制作用,尤其是对一些芽孢形成菌如蜡状芽孢杆菌、枯草芽孢杆菌、产气荚膜梭菌和生孢梭菌具有抑制作用,抑菌能力随发酵时间的延长而增强。提示在茶叶真菌发酵过程中,能够产生抑制食品致病菌和腐败菌的代谢物质。

关键词: 茯砖茶, 抑菌, 散囊菌, 致病菌

Abstract:

In this study, the microbial ecology and antimicrobial activity of Fuzhuan brick tea were investigated. The microbial separation and identification showed that Eurotium spp., Aspergillus spp. and Penicillium spp. were mainly responsible for the natural fermentation of Fuzhuan brick tea, and among them, Eurotium spp. was the dominant fungus. The water extract from Fuzhuan brick tea was found to have inhibitory effect on many food-borne pathogenic bacteria, especially on Bacillus cereus, Bacillus subtilis, Clostridium perfringens and Clostridium sporogenes, which displayed an ascent as the fermentation time was prolonged. This suggests the generation of metabolites able to inhibit food-borne pathogenic bacteria and spoilage bacteria during the fugal fermentation of Fuzhuan brick tea.

Key words: Fuzhuan brick tea, antibacterial activity, Erothium spp., food-borne pathogenic bacteria

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