食品科学 ›› 2010, Vol. 31 ›› Issue (24): 33-36.doi: 10.7506/spkx1002-6630-201024007

• 工艺技术 • 上一篇    下一篇

响应面法优化闪式提取牛蒡苷工艺优化

谢 捷,李红娟,朱兴一,杨 凡,王 平*   

  1. 浙江工业大学药学院,制药工程省部共建教育部重点实验室
  • 收稿日期:2010-02-08 修回日期:2010-08-03 出版日期:2010-12-25 发布日期:2010-12-29
  • 通讯作者: 王平 E-mail:pharmlab@zjut.edu.cn
  • 基金资助:

    浙江省国际合作项目(2007C14004)

Optimization of Homogenate Extraction for Arctiin from Arctium lappa L. by Response Surface Methodology

XIE Jie,LI Hong-juan,ZHU Xing-yi,YANG Fan,WANG Ping*   

  1. (Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences,
    Zhejiang University of Technology, Hangzhou 310014, China)
  • Received:2010-02-08 Revised:2010-08-03 Online:2010-12-25 Published:2010-12-29
  • Contact: WANG Ping E-mail:pharmlab@zjut.edu.cn

摘要:

采用响应面分析法优化牛蒡苷闪式提取的工艺条件。以牛蒡苷得率为指标,在单因素试验基础上,应用Box-Benhnken 中心组合设计试验,对提取工艺影响较大的乙醇体积分数、提取时间和液料比3 个工艺参数进行优化。最佳工艺条件为:乙醇体积分数83%、提取时间93s、液料比17:1(mL:g)、牛蒡子粒径≤ 1.0mm,在此工艺条件下,牛蒡苷得率为31.62mg/g,与预测值31.72mg/g 基本相符。闪式提取法是一种快速有效的提取牛蒡苷的方法。

关键词: 闪式提取, 牛蒡苷, 响应面分析法

Abstract:

Response surface analysis (RSA) methodology was used for optimizing homogenate extraction processing of arctiin from Arctium lappa L. Based on single-factor experiments, the effects of ethanol concentration, extraction time and liquid-material ratio on extraction rate of arctiin were explored by Box-Benhnken central composite design method. The optimal extraction conditions were ethanol concentration of 83%, extraction time of 93 s, liquid-material ratio of 17:1 (mL/g) and particle size of less than 1.0 mm. Under these optimal extraction conditions, the actual yield of total arctiin was 31.62 mg/g, which was consistent with the predicted yield of 31.72 mg/g. Results indicated that homogenate extraction was an effective method to extract arctiin from Arctium lappa L.

Key words: homogenate extraction, arctiin, response surface analysis (RSA)

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