食品科学 ›› 2011, Vol. 32 ›› Issue (7 ): 233-236.doi: 10.7506/spkx1002-6630-201107050

• 生物工程 • 上一篇    下一篇

花生根细胞悬浮培养制备白藜芦醇条件的优化

黄卫文1,黎继烈2,戴梓茹3,李忠海1   

  1. 1.中南林业科技大学食品科学与工程学院 2.中南林业科技大学生命科学与技术学院 3.钦州学院化学化工学院
  • 收稿日期:2010-06-07 修回日期:2011-02-13 出版日期:2011-04-15 发布日期:2011-03-30
  • 通讯作者: 黄卫文 E-mail:hww5626@163.com
  • 基金资助:
    湖南省教育厅科技项目(05C335)

Optimization of Suspension Culture Conditions for Preparing Resveratrol in Peanut Root Cells

HUANG Wei-wen1,LI Ji-lie2,DAI Zi-ru3,LI Zhong-hai1   

  1. 1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China;
    2. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;
    3. College of Chemistry and Chemical Engineering, Qinzhou University, Qinzhou 535000, China
  • Received:2010-06-07 Revised:2011-02-13 Online:2011-04-15 Published:2011-03-30

摘要: 以花生根愈伤组织为材料,采用二次通用旋转组合试验设计,优化花生根细胞悬浮培养产白藜芦醇的条件,探讨影响花生根细胞悬浮培养产白藜芦醇的最佳工艺参数。当摇床转速为130r/min、接种量为38.00g/L、pH5.80、蔗糖质量浓度为40.00g/L时,培养15d,花生根细胞增殖倍数最大为(4.71±0.04)倍,白藜芦醇产量达到907.3μg/g。

关键词: 二次通用旋转组合设计, 花生根细胞, 悬浮培养, 白藜芦醇

Abstract: Quadratic rotation combinatorial design was utilized to optimize suspension culture conditions for resveratrol production by peanut root callus tissue. Under the following optimized conditions: shaker rational speed 130 r/min; inoculum size 38.00 g/L; pH 5.80; and sucrose concentration 40.00 g/L, the maximum proliferation fold of suspension cells was 4.71 ± 0.04, and the resveratrol production reached 907.3 μg/g after 15-day incubation.

Key words: peanut root cell, callus, suspension culture, resveratrol

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