食品科学 ›› 2011, Vol. 32 ›› Issue (8): 194-196.doi: 10.7506/spkx1002-6630-201108044

• 分析检测 • 上一篇    下一篇

微波消解-可见分光光度法测定禽蛋中卵磷脂含量

葛庆联,吴 敏,张小燕,唐梦君,蒲俊华,高玉时,陈宽维*   

  1. 中国农科院家禽科学研究所
  • 出版日期:2011-04-25 发布日期:2011-04-12
  • 基金资助:
    国家公益性行业(农业)科研专项(nyhyzx03-46)

Microwave Digestion-Visible Spectrophotometric Determination of Lecithin in Eggs

GE Qing-lian,WU Min,ZHANG Xiao-yan,TANG Meng-jun,PU Jun-hua,GAO Yu-shi,CHEN Kuan-wei*   

  1. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China
  • Online:2011-04-25 Published:2011-04-12

摘要: 建立一种快速测定禽蛋中卵磷脂含量的方法-微波消解分光光度法。禽蛋匀浆液经过无水乙醇处理,去除其中磷蛋白对卵磷脂含量的干扰后,再采用微波消解法对禽蛋样品进行预处理后,用分光光度法于400nm波长处测定卵磷脂含量。方法回收率均在90%以上,变异系数均低于2%,具有良好的准确度与精密度。与传统方法相比,该方法具有试剂用量少,操作简单,减少沾污,改善工作环境等优点,是一种有效、实用的方法。

关键词: 微波消解, 卵磷脂, 禽蛋

Abstract: Microwave digestion coupled with visible spectrophotometry was used to establish an analytical method for the determination of lecithin content in eggs. Eggs were homogenized and treated with ethanol to remove the phosphate protein, Followed by microwave digestion. The detection of lecithin was spectrometriacally performed at 400 nm. The mean spike recovery rates of the established method for lecithin in eggs from three species of poultry were above 90% with a coefficient of variation of less than 2%. This method displayed excellent accuracy and precision, had many advantages over the conventional method, such as less reagent consumption, ease of operation and low environmental pollution. Therefore, it might be an effective and practical method.

Key words: visible spectrometry, lecithin, eggs

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