食品科学 ›› 2011, Vol. 32 ›› Issue (9): 156-158.doi: 10.7506/spkx1002-6630-201109035

• 生物工程 • 上一篇    下一篇

草鸡煲中腐败菌的分离鉴定

刘梦茵,刘 芳,诸永志,周 涛,*,徐为民   

  1. 1.南京师范大学金陵女子学院 2.江苏省农业科学院农产品加工研究所
  • 出版日期:2011-05-15 发布日期:2011-04-11
  • 基金资助:
    江苏省农业科技自主创新基金项目(CX(09)627;CX(10)230)

Isolation and Identification of Spoilage Bacteria in Native-chicken Soup

LIU Meng-yin1,2,LIU Fang2,ZHU Yong-zhi2,ZHOU Tao1,*,XU Wei-min2   

  1. 1. Ginling College, Nanjing Normal University, Nanjing 210097, China; 2. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 为了采取针对性措施控制草鸡煲中的微生物污染,采用平板划线分离方法对草鸡煲中的主要微生物菌群进行分离,再根据菌落形态、菌体形态、生理生化实验以及16S rDNA序列分析对菌株进行鉴定。结果表明,引起草鸡煲腐败变质的主要菌群有产气肠杆菌、成团泛生菌、假单胞菌、荧光假单胞菌、蜡状芽孢杆菌、类芽孢杆菌。

关键词: 草鸡煲, 细菌, 分离, 鉴定, 16S rDNA

Abstract: In order to achieve a specific strategy for controlling microbial contaminant of native-chicken soup, streak plate separation method was used to classify and identify the primary bacterial flora according to colony shape, bacterial shape, physio-biochemical tests as well as 16S rDNA sequence analysis. The results indicated that the primary bacteria in native-chicken soup were Enterobacter aerogenes, Pantoea agglomerans, Pseudomonas, Pseudomonas fluorescens, Bacillus cereus and Paenibacillus.

Key words: native-chicken soup, bacteria, isolation, identification, 16S rDNA gene

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