紫外诱变选育出芽短梗霉高产普鲁兰糖白化突变菌株

doi:10.7506/spkx1002-6630-201111040

食品科学 ›› 2011, Vol. 32 ›› Issue (11): 187-191.doi: 10.7506/spkx1002-6630-201111040

紫外诱变选育出芽短梗霉高产普鲁兰糖白化突变菌株

靳建忠,王慧娟,孔维甲,葛海涛,张宁,李炳学

1.沈阳农业大学土地与环境学院 2.沈阳农业大学生物科学技术学院

出版日期:2011-06-15 发布日期:2011-05-13
基金资助:
国家自然科学基金项目(31000674)；UNU-Kirin Fellowship Programme Follow-up Research(201101)

Breeding of a High Pullulan-producing Non-pigmented Mutant of Aureobasidium pullulans by UV-induced Mutagenesis

JIN Jian-zhong1，WANG Hui-juan1，KONG Wei-jia1，GE Hai-tao1，ZHANG Ning2，LI Bing-xue1,*

1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China； 2. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China

Online:2011-06-15 Published:2011-05-13

摘要/Abstract

摘要： 为了发酵获得无色素普鲁兰糖，对菌株Aureobasidium pullulan NG进行紫外诱变，成功获得一株高产普鲁兰糖的白化突变株UVMU3-1。将突变菌株与野生菌株比较发现，其菌体生长能力和分化能力未发生显著改变。突变菌株产出的多糖经红外光谱鉴定为普鲁兰糖，罐发酵实验表明突变菌株产糖能力强于野生菌株。在未经培养基和培养条件优化的情况下，罐发酵的糖转化率可以达到52.78%。突变株UVMU3-1可作为工业发酵普鲁兰糖的潜力菌株。

关键词: 紫外诱变, 出芽短梗霉, 普鲁兰糖

Abstract: A non-pigmented mutant having enhanced ability to produce pullulan was successfully obtained using Aureobasidium pullulan NG as the original strain by UV-induced mutagenesis and was named as UVMU3-1. Compared with the original strain, the mutant strain UVMU3-1 displayed similar capability of growth and differentiation. The polysaccharide produced by the mutant strain was identified as pullulan by infrared spectroscopy. Tank fermentation experiments showed that the pullulan-producing capability of the mutant strain was higher than that of the original strain and that the 72 h cultivation of 200 mL of seed culture of the mutant in 5 L of YDA culture medium provided a glucose conversion of 52.78%. In conclusion, UVMU3-1 is a potential strain for pullulan production in industrial fermentation.

Key words: UV mutagenesis, Aureobasidium pullulans, pullulan

中图分类号:

TS201.3

靳建忠，王慧娟，孔维甲，葛海涛，张宁，李炳学. 紫外诱变选育出芽短梗霉高产普鲁兰糖白化突变菌株[J]. 食品科学, 2011, 32(11): 187-191.

JIN Jian-zhong，WANG Hui-juan，KONG Wei-jia，GE Hai-tao，ZHANG Ning，LI Bing-xue. Breeding of a High Pullulan-producing Non-pigmented Mutant of Aureobasidium pullulans by UV-induced Mutagenesis[J]. FOOD SCIENCE, 2011, 32(11): 187-191.

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紫外诱变选育出芽短梗霉高产普鲁兰糖白化突变菌株

Breeding of a High Pullulan-producing Non-pigmented Mutant of Aureobasidium pullulans by UV-induced Mutagenesis

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