食品科学 ›› 2011, Vol. 32 ›› Issue (14): 152-155.doi: 10.7506/spkx1002-6630-201114031

• 工艺技术 • 上一篇    下一篇

芦笋下脚料皂苷超声提取工艺

孙 健1,2,李 曼3,王丽卫1,2,赵 兵1,*   

  1. 1.中国科学院过程工程研究所 生化工程国家重点实验室 2.中国科学院研究生院 3.北京城市学院生物医药学部
  • 出版日期:2011-07-25 发布日期:2011-06-18

Ultrasonic-assisted Extraction of Saponins from Asparagus officinalis Scraps

SUN Jian1,2,LI Man3,WANG Li-wei1,2,ZHAO Bing1,*   

  1. (1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China;3. Department of Biomedical, Beijing City University, Beijing 100083, China)
  • Online:2011-07-25 Published:2011-06-18

摘要: 采用超声强化技术对芦笋下脚料中的皂苷成分进行提取,通过两轮均匀试验对提取工艺进行优化。结果得到的最优提取工艺为液料比1:1、温度30℃、占空比0.4:1.6、功率200W、提取时间30min、pH5.05、酒精体积分数70%,该工艺条件下单次提取得率为7.5%(干基);两次提取累积回收率达到93.37%,3次提取累积回收率达到98.40%。在2L循环超声提取装置中的放大实验表明,两次提取累积回收率达到97.49%,3次可提取完全。表明超声强化方法适用于芦笋下脚料中皂苷的提取。

关键词: 芦笋, 皂苷, 均匀实验, 超声循环提取

Abstract: In order to improve the utilization rate of Asparagus officinalis L. scraps, ultrasonic assistance was applied for the extraction of saponins from the materials. After two rounds of uniform design, the optimal extraction conditions for achieving maximum extraction rate of saponins were material-to-liquid ratio of 1:1 (m/V), extraction temperature of 30 ℃, duty cycle of 0.4:1.6 (ratio of occupied to empty space), ultrasonic power of 200 W, extraction time of 30 min, extraction pH of 5.05 and ethanol concentration of 70%. Under the optimal conditions, the extraction rate of saponins was up to 7.5%, and the recovery rate was 98.78% after three rounds of extraction. The saponins could be fully recovered by three rounds of extractions in a 2 L circulated ultrasonic-assisted extraction device. Therefore, ultrasonic-assisted extraction is suitable for the extraction of saponins from Asparagus officinalis L. scraps.

Key words: Asparagus officinalis L., steroid saponins, uniform design, ultrasonic circulation extraction

中图分类号: