食品科学 ›› 2011, Vol. 32 ›› Issue (15): 191-195.doi: 10.7506/spkx1002-6630-201115043

• 生物工程 • 上一篇    下一篇

一株来自河南的雪莲菌TK3优势菌相分析

高 洁,张佳佳,顾丰颖,张小燕,苏忠锐,阮 晖,何国庆   

  1. 1.浙江大学生物系统工程与食品科学学院 2.海军医学研究所
  • 出版日期:2011-08-15 发布日期:2011-07-26
  • 基金资助:
    国家“863”计划项目(2006AA10Z316);国家自然科学基金项目(20776130); 浙江省科技计划基金项目(2009C32009)

Dominant Microflora Analysis of Tibetan Kefir Strain TK3 from Henan

GAO Jie1,ZHANG Jia-jia2,GU Feng-ying1,ZHANG Xiao-yan1,SU Zhong-rui1,RUAN Hui1,HE Guo-qing1,*   

  1. (1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; 2. The Naval Medical Research Institute, Shanghai 200433, China)
  • Online:2011-08-15 Published:2011-07-26

摘要: 对一株来自河南省的雪莲菌TK3进行初步菌相分析,扫描电镜结果表明:TK3是由乳酸菌及酵母菌构成的混菌共生体系,菌体附着在基质上,形成紧密不易分离的颗粒。对其菌相进行分离纯化得到6种优势菌,经镜检分析、生理生化分析及16S rDNA/26S rDNA鉴定,鉴定结果分别为:乳明串珠菌(Leuconostoc lactis)、乳酸乳球菌(Lactococcus lactis)、植物乳杆菌(Lactobacillus plantarum)、单孢酿酒酵母(Kazachstania unispora)、马克斯克鲁维酵母(Kluyveromyces marxianus)以及exigua酿酒酵母(Kazachstania exigua),在发酵乳中存在的数量级分别为107、107、107、106、106、107CFU/mL,并且在4℃存放7d后各个菌群数量仍保持稳定。

关键词: 雪莲菌, 菌相分析, 扫描电镜

Abstract: Microflora analysis of Tibetan kefir strain TK3 from Henan was conducted in this paper. The microstructure was observed under a scanning electron microscope. The strain TK3 was composed of Lactobacillus and yeast, and attached to the matrix. Physiological and biochemical tests, and partial 16S rDNA (bacteria) and 26S rDNA (yeast) sequence identification revealed that the dominant microbial species in TK3 were Leuconostoc lactis, Lactococcus lactis, Lactobacillus plantarum, Kazachstania unispora, Kluyveromyces marxianus, and Kazachstania exigua, which presented in fermented milk in the order of magnitude of 107, 107, 107, 106, 106 CFU/mL and 107 CFU/mL, respectively. Moreover, the numbers of these microbial species remained stable after 7 days of storage at 4 ℃.

Key words: Tibetan kefir, microflora analysis, scanning electron microscope

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