乳源酪蛋白糖巨肽对溃疡性结肠炎小鼠MUC2表达的影响

doi:10.7506/spkx1002-6630-201115051

食品科学 ›› 2011, Vol. 32 ›› Issue (15): 230-233.doi: 10.7506/spkx1002-6630-201115051

乳源酪蛋白糖巨肽对溃疡性结肠炎小鼠MUC2表达的影响

贾玉臣，陈庆森*，冯毅楠，徐怡君，李洋

天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院

出版日期:2011-08-15 发布日期:2011-07-26
基金资助:
国家自然科学基金项目(30771524；31071522)

Effect of Bovine Casein Glycomacropeptide on MUC2 Expression in Mice with Ulcerative Colitis

JIA Yu-chen，CHEN Qing-sen*，FENG Yi-nan，XU Yi-jun，LI Yang

(Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China)

Online:2011-08-15 Published:2011-07-26

摘要/Abstract

摘要： 目的：研究酪蛋白糖巨肽(CGMP)对溃疡性结肠炎 (UC)小鼠结肠黏蛋白MUC2表达的影响，从而进一步探讨CGMP对UC的改善效果。方法：Balb/c 实验小鼠随机分为正常对照组、模型对照组、CGMP组和SASP(柳氮磺胺吡啶)治疗组。对模型对照组、CGMP组和SASP组利用OXZ灌肠方法建立UC模型，诱导建模后同时给予CGMP组灌胃CGMP溶液(剂量为50mg/(kg ·d))以及SASP组灌胃SASP溶液(剂量为40mg/(kg ·d))，连续灌胃5d。期间记录小鼠每天体质量变化，进行疾病活动指数 (DAI)，末次灌胃后处死小鼠，分离结肠进行组织损伤评分并制作石蜡切片，分别进行HE染色和MUC2免疫组化染色。结果：CGMP可有效缓解溃疡性结肠炎小鼠的一般状态，同时显著降低小鼠结肠的大体形态损伤指数，HE染色结果表明CGMP可以维持小鼠肠黏膜上皮细胞的完整，免疫组化结果证实了CGMP可以显著促进小鼠肠黏膜MUC2的分泌。结论：CGMP可以显著促进小鼠结肠MUC2的表达，通过维系肠黏膜的屏障作用来减缓炎症。

关键词: 酪蛋白糖巨肽, CGMP, 溃疡性结肠炎, MUC2

Abstract: Objective: The effect of bovine casein glycomacropeptide (CGMP) on MUC2 expression in mice with ulcerative colitis (UC) was investigated to explore the therapeutic efficacy of CGMP for UC. Methods: Mice were randomly divided into four groups including normal control group, model control group, CGMP group and sulfasalazine (SASP) group. UC model establishment was carried out by enema administration of oxazolone. The mice in the model control and normal control groups were given regular feed, and the mice in the CGMP group and SASP group were administered with CGMP at the dose of 50 mg/(kg ·d) and SASP at the dose of 40 mg/(kg ·d) after for 5 consecutive days, respectively. The changes in body weights and disease activity index (DAI) were recorded every day. The colon was separated to conduct HE staining and MUC2 immunohistochemical staining and record visible lesion score. Results: CGMP could effectively alleviate ulcerative colitis and reduce visible lesions in the mouse colon. HE staining revealed integrated epithelial cells in the colon of CGMP-treated mice. Meanwhile, IHC staining confirmed that CGMP could promote the secretion of MUC2 in the mouse colon. Conclusion: CGMP can alleviate colon inflammation by promoting the secretion of MUC2 in the mouse colon to enhance the barrier protection of intestinal mucosa.

Key words: casein glycomacropeptide(CGMP), ulcerative colitis, MUC2

中图分类号:

TS252.1

贾玉臣，陈庆森，冯毅楠，徐怡君，李洋. 乳源酪蛋白糖巨肽对溃疡性结肠炎小鼠MUC2表达的影响[J]. 食品科学, 2011, 32(15): 230-233.

JIA Yu-chen，CHEN Qing-sen，FENG Yi-nan，XU Yi-jun，LI Yang. Effect of Bovine Casein Glycomacropeptide on MUC2 Expression in Mice with Ulcerative Colitis[J]. FOOD SCIENCE, 2011, 32(15): 230-233.

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乳源酪蛋白糖巨肽对溃疡性结肠炎小鼠MUC2表达的影响

Effect of Bovine Casein Glycomacropeptide on MUC2 Expression in Mice with Ulcerative Colitis

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