食品科学 ›› 2011, Vol. 32 ›› Issue (16): 80-84.doi: 10.7506/spkx1002-6630-201116017

• 工艺技术 • 上一篇    下一篇

超声波辅助提取杜仲翅果桃叶珊瑚甙工艺优化

马 龙,姚 晗,孙兰萍,许 晖   

  1. 蚌埠学院生物与食品工程系
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    安徽省高等学校自然科学研究重点项目(KJ2011Z240); 安徽省高等学校省级食品科学与工程特色专业建设点资助项目(20101091);安徽省食品科学与工程教学团队项目(20101094)

Process Optimization for Ultrasonic-assisted Extraction of Aucubin from Eucommia ulmoides Oliv. Key Fruits

MA Long,YAO Han,SUN Lan-ping,XU Hui   

  1. (Department of Biotechnology and Food Engineering, Bengbu College, Bengbu 233000, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 目的:研究超声波辅助提取杜仲翅果中桃叶珊瑚甙的较佳工艺条件,为杜仲翅果资源的综合开发利用提供参考。方法:在单因素试验的基础上,通过Box-Behnken试验设计确定乙醇体积分数、超声波功率、提取时间及液料比等因素的最佳工艺条件。结果:超声波辅助提取杜仲翅果中桃叶珊瑚甙的最佳提取工艺条件为乙醇体积分数72.1%、超声波功率300W,提取时间20.5min、液料比12.3:1(mL/g),在该条件下杜仲翅果桃叶珊瑚甙的实际提取率为5.91%。在试验范围内各因素对桃叶珊瑚甙得率影响大小依次为乙醇体积分数>料液比>提取时间。结论:超声波辅助提取法能够较好地提取杜仲翅果桃叶珊瑚甙。

关键词: 杜仲翅果, 桃叶珊瑚甙, 超声波辅助提取, 响应曲面法

Abstract: Objective: To optimize the conditions for ultrasonic-assisted extraction of aucubin from Eucommia ulmoides Oliv. key fruits. Methods: One-factor-at-a-time method and response surface methodology based on Box-Behnken experimental design were used for process conditions optimization. Results: The optimal extraction conditions were 72.1% aqueous ethanol solution as the extraction solvent, ultrasonic power of 300 W, extraction time of 20.5 min and solid/liquid ratio of 12.3:1 (mL/g). Under these conditions, the extraction yield of aucubin was observed to be 5.91%. Ethanol concentration had the largest impact on the extraction yield of aucubin, followed by solid/liquid ratio and extraction time. Conclusion: Ultrasonic assistance can enhance the extraction of aucubin from Eucommia ulmoides Oliv. key fruits.

Key words: Eucommia ulmoides Oliv. key fruits, aucubin, ultrasonic wave-assisted extraction, response surface methodology

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