食品科学 ›› 2011, Vol. 32 ›› Issue (16): 89-92.doi: 10.7506/spkx1002-6630-201116019

• 工艺技术 • 上一篇    下一篇

超声协同双水相体系提取路边青总多酚工艺

欧阳玉祝1,2,张辞海2,薛 慧2,吴道宏2   

  1. 1.林产化工工程湖南省重点实验室 2.吉首大学食品科学研究所
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    林产化工工程湖南省重点实验室开放基金资助项目(JDZ201107)

Ultrasonic-assisted Two-phase Aqueous Extraction of Total Polyphenols from the Shoot of Geum japonicum Thunb. var. chinense F. Bolle

OUYANG Yu-zhu1,2,ZHANG Ci-hai2,XUE Hui2,WU Dao-hong2   

  1. (1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Zhangjiajie 416000, China; 2. Institute of Food Science, Jishou University, Jishou 416000, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 采用超声协同乙醇-铵盐双水相体系提取路边青总多酚,通过单因素和正交试验考查料液比、乙醇初始体积与水初始体积比、提取温度和提取时间对总多酚提取率的影响。结果表明:最佳工艺为料液比1:20(g/mL)、乙醇初始体积与水初始体积比0.8、硫酸铵质量浓度0.27g/mL、提取温度60℃、超声提取时间60min,总多酚提取率为3.04%。本法是一种提取效率高,时间短,能耗低,不影响提取物活性的新工艺。

关键词: 双水相体系, 超声波, 路边青, 总多酚

Abstract: The shoot of Geum japonicum Thunb. var. chinense F. Bolle was ultrasonified in ethanol-ammonium salt two-phase aqueous system to accelerate the extraction of total polyphenols. Operating parameters such as solid-to-liquid ratio, volume ratio of initial ethanol to initial water, temperature and extraction time were optimized by one-factor-at-a-time and orthogonal array design methods for maximizing the extraction yield of total polyphenols. Solid-to-liquid ratio of 1:20 (g/mL), volume ratio of initial ethanol to initial water of 0.8, ammonium sulfate concentration of 0.27g/mL, extraction temperature of 60 ℃ and extraction time of 60 min were found optimal. Under the optimal extraction conditions, the extraction yield of total polyphenols was 3.04%. This method can provide a novel approach for the extraction of total polyphenols with highly efficient, time-saving and energy-saving benefits and no impact on the biological activity of extracts.

Key words: two-phase aqueous system, ultrasonic, Geum japonicum Thunb. var. chinense F. Bolle, total polyphenols

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