食品科学 ›› 2011, Vol. 32 ›› Issue (16): 103-107.doi: 10.7506/spkx1002-6630-201116022

• 工艺技术 • 上一篇    下一篇

青钱柳愈伤组织总黄酮碱溶酸沉提取工艺研究

陈继光,米丽雪,上官新晨*,李 翔,钟世根   

  1. 江西农业大学食品科学与工程学院 天然产物研究与开发重点实验室
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    江西省主要学科学术和技术带头人项目(2009DD00900);江西省科技厅支撑项目(2009BSB09100)

Optimization of Alkali Extraction and Acid Precipitation Process for Total Flavonoids from Cyclocarya paliurus Callus

CHEN Ji-guang,MI Li-xue,SHANGGUAN Xin-chen*,LI Xiang,ZHONG Shi-gen   

  1. (Key Laboratory of Natural Products Research and Development, College of Food Science and Engineering, Jiangxi Agricultural University,Nanchang 330045,China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 目的:研究以碱溶酸沉法提取青钱柳愈伤组织中的总黄酮。方法:采用碱溶酸沉法,对影响青钱柳愈伤组织中总黄酮提取的pH值、固液比、提取时间、硼砂用量等因素进行系统研究。结果:青钱柳愈伤组织中总黄酮碱提酸沉的最佳提取工艺为:采用固液比1:20,碱提液pH9,硼砂含量2.5%,提取时间60min,酸沉pH 4.5。结论:在最佳提取工艺下,青钱柳愈伤组织中总黄酮得率达到4.01%。

关键词: 青钱柳, 愈伤组织, 总黄酮, 碱溶酸沉

Abstract: Objective: To optimize the alkali extraction and acid precipitation of total flavonoids from Cyclocarya paliurus callus. Methods: The effects of pH, material/liquid ratio, borax amount and extraction time on the extraction rate of total flavonoids from Cyclocarya paliurus callus. Results: The optimal extraction conditions were extraction solvent pH of 9.0, borax amount of 2.5%, extraction time of 60 min, and acid precipitation pH of 4.5. Conclusion: Under the optimal conditions, the extraction rate of total flavonoids was up to 4.01%.

Key words: Cyclocarya paliurus, callus, total flavonoids, alkali extraction and acid precipitation

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