食品科学 ›› 2011, Vol. 32 ›› Issue (16): 187-191.doi: 10.7506/spkx1002-6630-201116039

• 工艺技术 • 上一篇    下一篇

大孔吸附树脂吸附分离高活性玉米抗氧化肽

刁静静1,曹龙奎1,2,*   

  1. 1. 黑龙江省农产品加工工程技术研究中心
    2.黑龙江八一农垦大学食品学院
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    大庆市高新区创新基金项目(DQGX08YF007)

Separation of Antioxidant Peptides from Maize by Macroporous Resin Adsorption

DIAO Jing-jing1,CAO Long-kui1,2,*   

  1. (1. Agri-food Processing Development Centre of Heilongjiang, Daqing 163319, China; 2. College of Food Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 采用大孔吸附树脂对玉米抗氧化肽进行分离,通过单因素试验得出XAD-7HP树脂为最适树脂,在此基础上,研究不同pH值条件下的静态吸附能力和不同解吸剂的静态解吸能力等实验,确定玉米抗氧化肽吸附分离的基本参数为pH7.0、解吸剂体积分数70%。通过动态吸附分离实验得出,该树脂可以达到分离纯化玉米抗氧化肽的目的,而且分离后的玉米肽的抗氧化活性比原液提高了1倍。

关键词: 大孔吸附树脂, 玉米抗氧化肽, 静态吸附, 解吸

Abstract: In this study, three types of macroporous resin were assessed for their adsorption and desorption capacity towards antioxidant peptides in the maize protein hydrolysate, prepared by alcalase hydrolysis, and XAD-7HP resin was found to be the most suitable resin among them. The static adsorption of XAD-7HP resin and the static desportion of antioxidant peptides with different desorption solvents were performed under different pH conditions. As a result, the optimal process parameters for the separation of antioxidant peptides were sample pH 7.0 and 70% aqueous ethanol as the desorption solvent. Antioxidant maize peptides were successfully separated by the dynamic adsorption of XAD-7HP resin, resulting in a 2-fold increase in DPPH free radical scavenging activity.

Key words: macroporous resin, antioxidant maize peptides, static adsorption, desorption capacity

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