食品科学 ›› 2011, Vol. 32 ›› Issue (16): 249-252.doi: 10.7506/spkx1002-6630-201116053

• 分析检测 • 上一篇    下一篇

不同品种荔枝果实游离氨基酸分析

杨苞梅1,姚丽贤1,国 彬1,何兆桓1,李国良1,周昌敏1,涂仕华2   

  1. 1.广东省农业科学院土壤肥料研究所,广东省养分资源循环利用与耕地保育重点实验室 2.国际植物营养研究所成都办事处
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    国家现代农业产业技术体系建设专项(nycytx-32);国际植物营养研究所资助项目

Analysis of Free Amino Acids in Litchi Fruits from Different Cultivars

YANG Bao-mei1,YAO Li-xian1,GUO Bin1,HE Zhao-huan1,LI Guo-liang1,ZHOU Chang-min1,TU Shi-hua2   

  1. (1. Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Soil and Fertilizer Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2. Chengdu Office of International Plant Nutrition Institute, Chengdu 610066, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 为探明荔枝果实中游离氨基酸组成及含量,采用AccQ ·Tag柱前衍生反相高效液相色谱法测定紫娘喜、大丁香、黑叶、兰竹和甜眼荔枝果实中的17种游离氨基酸含量。结果表明:测定方法重现性好,精密度高;甜眼果实中的人体必需氨基酸含量占氨基酸总量的36.4%,人体必需氨基酸含量与非必需氨基酸含量之比为0.57,较接近理想蛋白质的标准要求;紫娘喜、大丁香、黑叶、兰竹和甜眼荔枝果实均含有丰富的药效氨基酸、鲜味氨基酸、甜味氨基酸以及少量的芳香族氨基酸,但不同品种间的含量及比例差异较大。因此,不同荔枝品种的口感和风味明显不同。

关键词: 反相高效液相色谱法, 6-氨基喹啉基-N-羟基琥珀酰亚胺基-氨基甲酸酯(AQC), 氨基酸, 荔枝

Abstract: An AccQ ·tag precolumn derivatization and reversed phase HPLC method was presented to determine the contents of 17 free amino acids in litchi fruits from 5 different cultivars, Ziniangxi, Dadingxiang, Heiye, Lanzhu and Tianyan. The method proved highly reproducible and precise. The ratio of essential amino acids to total amino acids was 36.4% and to non-essential amino acids 0.57 in Tianyan, indicating good agreement with the ideal human protein requirements. The five litchi cultivars were all rich in medicinal, umami and sweet amino acids with a difference in their contents and small amounts of several aromatic amino acids were also found. Therefore, different litchi cultivars distinctly differ in taste and flavor.

Key words: reversed phase high performance liquid chromatography, AQC, amino acids, litchi

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