食品科学 ›› 2011, Vol. 32 ›› Issue (17): 246-249.doi: 10.7506/spkx1002-6630-201117051

• 生物工程 • 上一篇    下一篇

产核糖核酸酶PA110-7菌株的筛选与初步鉴定

矫玉翠,生吉萍,郑嫣燕,王正荣,葛 佳,申 琳*   

  1. 中国农业大学食品科学与营养工程学院
  • 发布日期:2011-08-30
  • 基金资助:
    国家公益性行业(农业)科研专项(200803033)

Isolation and Primary Identification of Ribonuclease-producing Strain PA 110-7 from Suaedae glaucae Bunge

JIAO Yu-cui,SHENG Ji-ping,ZHENG Yan-yan,WANG Zheng-rong,GE Jia,SHEN Lin*   

  1. (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Published:2011-08-30

摘要: 为筛选产核糖核酸酶并且具有抗病毒活性的植物来源的菌株,并鉴定。本实验从健康的碱蓬中分离到50株益生菌,通过平板显色法筛选得到9株产核糖核酸酶的益生菌,通过噬菌斑法进行复筛得到抗噬菌体率高达69%的菌株110-7。根据菌株的菌落形态、大小、颜色,革兰氏染色、芽孢染色等观察菌体形态以及生理生化实验、16S rDNA序列测定进行鉴定,初步判定该菌株为苏云金芽孢杆菌(Bacillus thuringiensis)。

关键词: 盐生植物, 益生菌, 核糖核酸酶, 分离, 鉴定

Abstract: In order to screen and identify ribonuclease-producing strain and evaluate its antiviral activity, 50 probiotics from healthy Suaedae glaucae Bunge were isolated and 9 ribonuclease-producing strains were screened by color plate method. The inhibitory activity of strain PA110-7 against Escherichia coli was 69%. Based on the analyses of colony morphology, size and color, Gram staining, spore staining, physiological-biochemical characterization and 16S rDNA sequencing, strain PA110-7 was identified as Bacillus thuringiensis.

Key words: Suaedae glaucae Bunge, probiotics, ribonuclease, isolation, identification

中图分类号: