大孔树脂筛分马兰中活性组分及体外抗脂质过氧化测定

doi:10.7506/spkx1002-6630-201122019

食品科学 ›› 2011, Vol. 32 ›› Issue (22): 89-94.doi: 10.7506/spkx1002-6630-201122019

大孔树脂筛分马兰中活性组分及体外抗脂质过氧化测定

刘新明，潘道东，吕丽爽*

南京师范大学金陵女子学院食品科学与工程系

出版日期:2011-11-25 发布日期:2011-11-11

Purification of Bioactive Compounds Resulting from Sequential Organic Solvent Extraction of Kalimeris indica (L.) Sch-Bip by Macroporous Resin Adsorption and Determination of in vitro Lipid Peroxidation Inhibitory Activity

LIU Xin-ming，PAN Dao-dong，LU . . Li-shuang*

(Department of Food Science and Technology, Ginling College, Nanjing Normal University, Nanjing 210097, China)

Online:2011-11-25 Published:2011-11-11

摘要/Abstract

摘要： 通过大孔树脂富集马兰粗提液中黄酮及三萜类化合物并对其进行体外抗脂质过氧化测定。比较了15种树脂的静态吸附和解吸附，以及吸附动力学、吸附等温线等性能，从中筛选出兼具富集黄酮类和三萜类化合物的最佳树脂AB-8。进而选择AB-8柱动态富集马兰中活性组分并对其进行活性组分的抗脂质过氧化测定。通过不同体积分数乙醇溶液洗脱显示：20%洗脱组分中黄酮类化合物含量最高，达到62.7%，同时含有19%的三萜类化合物，其总得率达到51.5%，60%洗脱组分中三萜类化合物含量最高，达到58.5%，总得率达到22.3%。同时，对所得主要组分进行抑制过氧化脂质活性实验，结果表明：两主要组分对体外组织(心、肝、肾、脑)的脂质过氧化均有一定的抑制效果，其中对肝脏的抑制效果最为明显。

关键词: 大孔吸附树脂, 马兰, 三萜类化合物, 黄酮类化合物

Abstract: Macroporous resin adsorption was used to purify flavonoids and triterpenoids from crude extract resulting from sequential organic solvent extraction of the whole plant of Kalimeris indica (L.) Sch-Bip. with 80% ethanol, petroleum ether (decolorization) and ethyl acetate and their lipid peroxidation inhibitory activity in vitro was measured. AB-8 was selected as the best resin out of 15 ones by comparing their static adsorption and desorption, adsorption kinetic characteristics and isothermal adsorption curves. Kinetic adsorption and desorption on AB-8 column were used to purify flavonoids and triterpenoids. The results of elution with different concentrations of ethanol showed that 20% ethanol eluate contained the largest amount of flavonoids, reaching 62.7%, and 19% triterpenoids with a total yield of 51.5% and that 60% ethanol eluate had the highest triterpenoid content, reaching 58.5% with a total yield of 22.3%. Both constituents had some inhibitory effect on lipid peroxidation in mouse heart, liver, kidney and brain tissues in vitro, especially on the liver.

Key words: macro-porous resin, Kalimeris indica (L.) Sch-Bip, triterpenoids, flavonoids, lipid peroxidation

中图分类号:

TS255

刘新明，潘道东，吕丽爽. 大孔树脂筛分马兰中活性组分及体外抗脂质过氧化测定[J]. 食品科学, 2011, 32(22): 89-94.

LIUXin-ming，PANDao-dong，LU..Li-shuang. Purification of Bioactive Compounds Resulting from Sequential Organic Solvent Extraction of Kalimeris indica (L.) Sch-Bip by Macroporous Resin Adsorption and Determination of in vitro Lipid Peroxidation Inhibitory Activity[J]. FOOD SCIENCE, 2011, 32(22): 89-94.

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大孔树脂筛分马兰中活性组分及体外抗脂质过氧化测定

Purification of Bioactive Compounds Resulting from Sequential Organic Solvent Extraction of Kalimeris indica (L.) Sch-Bip by Macroporous Resin Adsorption and Determination of in vitro Lipid Peroxidation Inhibitory Activity

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