凝胶渗透色谱-高效液相色谱联用测定食用植物油中苯并(a)芘

doi:10.7506/spkx1002-6630-201122043

食品科学 ›› 2011, Vol. 32 ›› Issue (22): 209-212.doi: 10.7506/spkx1002-6630-201122043

凝胶渗透色谱-高效液相色谱联用测定食用植物油中苯并(a)芘

郑睿行，祝华明*，龚鸿萍，方芳，张旭，方建军

衢州市质量技术监督检测中心

出版日期:2011-11-25 发布日期:2011-11-11

Determination of Benzo(a)Pyrene in Edible Vegetable Oils by HPLC-GPC

ZHENG Rui-hang，ZHU Hua-ming*，GONG Hong-ping，FANG Fang，ZHANG Xu，FANG Jian-jun

(Quzhou Municipal Quality and Technical Supervision and Testing Center, Quzhou 324002, China)

Online:2011-11-25 Published:2011-11-11

摘要/Abstract

摘要： 为建立凝胶渗透色谱-高效液相色谱联用技术测定食用植物油中苯并(a)芘残留量的分析方法，采用凝胶渗透色谱进行样品前处理，用反相高效液相色谱荧光法对样品中苯并(a)芘残留量进行测定，以保留时间定性、外标法定量。结果表明，苯并(a)芘在0～20μg/L范围内，其线性相关系数是0.9998，植物油样品的最低检出限为0.1μg/kg，加标回收率为88.3%～94.4%，相对标准偏差为2.3%～4.9%。该方法灵敏度高、准确度好，对5种不同浓度质控样品进行测试分析，均获得满意结果，说明本方法适用于食用植物油中苯并(a)芘的快速测定。

关键词: 反相高效液相色谱, 食用植物油, 苯并(a)芘, 凝胶渗透色谱

Abstract: A gel permeation chromatography-phase high performance liquid chromatography (GPC-HPLC) was developed to determine benzo(a)pyrene residues in edible vegetable oils. In this method, sample pretreatment was achieved using gel permeation chromatography (GPC), and benzo(a)pyrene residues in samples were determined by reversed-phase high performance liquid chromatography (RP-HPLC) with fluorescence detection using retention time for qualitative analysis and external standards for quantitative analysis. The results showed that over the range from 0 to 20 μg/L, the correlation coefficient of benzo(a)pyrene was 0.9998. The detection limit of benzo(a)pyrene in edible vegetable oil was 0.1 μg/kg. The mean spike recoveries of benzo(a)pyrene were from 88.3% to 94.4% across three spike levels with a relative standard deviation (RSDs) ranging from 2.3% to 4.9%. The sensitive and accurate method allowed the determination of benzo(a)pyrene in 5 different quality control samples with satisfying results, suggesting its good applicability in practice.

Key words: edible vegetable oil, benzo(a)pyrene, gel permeation chromatography (GPC), reversed-phase high performance liquid chromatography (RP-HPLC)

中图分类号:

TS207.3

郑睿行，祝华明，龚鸿萍，方芳，张旭，方建军. 凝胶渗透色谱-高效液相色谱联用测定食用植物油中苯并(a)芘[J]. 食品科学, 2011, 32(22): 209-212.

ZHENGRui-hang，ZHUHua-ming，GONGHong-ping，FANGFang，ZHANGXu，FANGJian-jun. Determination of Benzo(a)Pyrene in Edible Vegetable Oils by HPLC-GPC[J]. FOOD SCIENCE, 2011, 32(22): 209-212.

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凝胶渗透色谱-高效液相色谱联用测定食用植物油中苯并(a)芘

Determination of Benzo(a)Pyrene in Edible Vegetable Oils by HPLC-GPC

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