食品科学 ›› 2011, Vol. 32 ›› Issue (24): 182-185.doi: 10.7506/spkx1002-6630-201124038

• 工艺技术 • 上一篇    下一篇

猪骨粉酶解工艺优化

左勇1,冯治平1,边名鸿1,谢晖1,鞠帅1,李杨1,刘达玉2   

  1. 1.四川理工学院生物工程学院 2.成都大学 肉类加工四川省重点实验室
  • 出版日期:2011-12-25 发布日期:2011-12-29
  • 基金资助:
    肉类加工四川省肉类加工重点实验室项目(10R07)

Process Optimization for Enzymatic Hydrolysis of Pig Bone Powder

ZUO Yong1,FENG Zhi-ping1,BIAN Ming-hong1,XIE Hui1,JU Shuai1,LI Yang1,LIU Da-yu2   

  1. (1. College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, China; 2. Liquor-Making Biotech and Application Key Laboratory of Sichuan Province, Chengdu University, Chengdu 610106, China)
  • Online:2011-12-25 Published:2011-12-29

摘要: 对猪骨粉进行酶解,探讨酶的种类、温度、时间、底物质量分数和pH值对酶解效果的影响。在单因素试验的基础上,对酶解温度、时间、底物质量分数和pH值4因素进行正交试验。结果表明:选用木瓜蛋白酶、酶用量10000U/g、在55℃、底物质量分数5%、pH8的条件下酶解4h,酶解效果最好。

关键词: 骨粉, 酶解条件, 三氯乙酸氮溶解指数, 苦味值

Abstract: The enzymatic hydrolysis of pig bone powder was optimized with respect to enzyme type, temperature, time, substrate concentration and pH by one-factor-at-a-time method in combination with orthogonal array method. The best hydrolysis results were achieved by using papain to hydrolyze pig bone powder for 4 h at pH 8, 55 ℃ and an enzyme dosage of 10000 U/g.

Key words: pig bone powder, enzymatic hydrolysis conditions, TCA-NSI (trichloroacetic acid-nitrogen solution index), bitterness

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