食品科学 ›› 2012, Vol. 33 ›› Issue (4): 100-103.doi: 10.7506/spkx1002-6630-201204021

• 工艺技术 • 上一篇    下一篇

响应面优化超声-微波协同辅助提取茶多糖工艺

陈义勇,窦祥龙,黄友如,朱东兴,郁 达   

  1. 1.常熟理工学院生物与食品工程学院 2.常熟海虞茶叶有限公司
  • 出版日期:2012-02-25 发布日期:2012-02-14
  • 基金资助:
    常熟市2010 年农业科技计划项目(CN201014)

Optimization of Ultrasonic/Microwave-Assisted Extraction of Polysaccharides from Green Tea by Response Surface Methodology

CHEN Yi-yong,DOU Xiang-long,HUANG You-ru,ZHU Dong-xing,YU Da   

  1. 1. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China; 2. Changshu Haiyu Tea Co. Ltd., Changshu 215500, China
  • Online:2012-02-25 Published:2012-02-14

摘要: 以水作为提取溶剂,粗绿茶作为原料,通过响应面优化超声-微波协同辅助提取茶多糖的最佳工艺条件,比较传统水浴浸提法和超声-微波协同辅助提取法对茶多糖得率、纯度和结构的影响。结果表明:超声-微波协同辅助提取茶多糖的最佳工艺条件为提取时间23min、料液比1:30(g/mL)、微波功率90W。与传统的水浴浸提法相比,超声-微波协同辅助提取法在较短的超声提取时间下,茶多糖的得率从2.95%提高到4.19%,纯度从70.15%提高到86.08%,两种提取方法所得的茶多糖基团基本相同。

关键词: 超声-微波协同辅助提取, 传统水浴浸提法, 茶多糖

Abstract: Response surface methodology was used to optimize the ultrasonic/microwave-assisted extraction of water-soluble polysaccharides from crude green tea. The traditional water extraction and ultrasonic/microwave-assisted extraction methods were compared for their effect on extraction efficiency, purity and structure of polysaccharides. The optimal ultrasonic-assisted extraction conditions were extraction time of 23 min, material-to-liquid ratio of 1:30 (g/mL) and microwave power of 90 W. The ultrasonic/microwave-assisted extraction method required shorter time and increased polysaccharide yield from 2.95% to 4.19% and polysaccharide purity from 70.15% to 86.08% compared with the traditional water extraction method. Polysaccharides extracted by the methods had basically the same groups.

Key words: ultrasonic/microwave-assisted extraction, traditional water bath extraction, polysaccharides from green tea

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