食品科学 ›› 2012, Vol. 33 ›› Issue (4): 109-114.doi: 10.7506/spkx1002-6630-201204023

• 工艺技术 • 上一篇    下一篇

响应面法优化脐橙渣中水溶性膳食纤维提取工艺

吴笑臣,王科军,钟金莲,罗序中   

  1. 赣南师范学院化学与生命科学学院
  • 出版日期:2012-02-25 发布日期:2012-02-14
  • 基金资助:
    江西省科技厅科技支撑计划项目(2010BNB1400);江西省教育厅科学技术研究项目(GJJ10587); 江西省教育厅青年科学基金项目(GJJ11214);赣州市果业基金项目(GZGY2008-1); 赣南师范学院自然科学研究课题(09kyz10)

Response Surface Methodology for Optimization of Extraction Process of Soluble Dietary Fiber from Navel Orange Residue

WU Xiao-chen,WANG Ke-jun,ZHONG Jin-lian,LUO Xu-zhong   

  1. College of Chemistry and Life Science, Gannan Normal University, Ganzhou 341000, China
  • Online:2012-02-25 Published:2012-02-14

摘要: 以赣南脐橙渣为原料,采用纤维素酶制备水溶性膳食纤维。在单因素试验基础上,选取酶解温度、加酶量(质量分数)和酶解时间为响应变量,以水溶性膳食纤维得率为响应值,利用Box-Behnken试验设计方案和响应面分析法,建立水溶性膳食纤维得率与响应变量的回归方程,并确定最佳提取条件为酶解温度48℃、加酶量1.25%、酶解时间5h,此条件下水溶性膳食纤维得率为13.11%,与预测值13.14%较为一致。

关键词: 脐橙渣, 水溶性膳食纤维, 响应面分析法, 优化

Abstract: Navel orange residue was treated with cellulase to extract soluble dietary fiber. Extraction parameters including extraction temperature, cellulase dose and length of extraction time were optimized using Box-Behnken design and response surface methodology based on single factor investigations for achieving maximum yield of soluble dietary fiber. A regression equation reflecting the relationship between the yield of soluble dietary fiber and the extraction parameters was set up. The optimal extraction parameters were found as follows: extraction temperature, 48 ℃; cellulase dose, 1.25%; length of extraction time, 5 h. Under the optimized conditions, the actual yield of soluble dietary fiber was 13.11%, close to the predicted value of 13.14%.

Key words: navel orange residue, soluble dietary fiber, response surface methodology, optimization

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