食品科学 ›› 2012, Vol. 33 ›› Issue (7): 182-187.doi: 10.7506/spkx1002-6630-201207039

• 生物工程 • 上一篇    下一篇

植物乳杆菌YSQ 规模化生产的低成本培养基配方优化

胡文锋1,张春丽1,2,*,刘秉杰3,杨益衡3,罗文华1,李雪玲1   

  1. 1.华南农业大学食品学院 2.佛山南海润佛生物有限公司 3.东莞市石龙津威饮料食品有限公司
  • 出版日期:2012-04-15 发布日期:2012-04-20
  • 基金资助:
    广东省教育部产学研结合计划项目(2010B09400038);广东省科技厅工业攻关重大项目(2008A010900003); 东莞市科技局高等院校科研机构科技计划项目(2007108101105)

Optimization of Low-Cost Culture Medium for Large-Scale Production of Lactobacillus plantarum YSQ

HU Wen-feng1,ZHANG Chun-li1,2,*,LIU Bing-jie3,YANG Yi-heng3,LUO Wen-hua1,LI Xue-ling1   

  1. (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. Foshan Nanhai Runfor Biology Co. Ltd., Foshan 528231, China;3. Dongguan Shilong Jingwei Beverage and Food Co. Ltd., Dongguan 523320, China)
  • Online:2012-04-15 Published:2012-04-20

摘要: 通过单因素试验、Plackett-Burman试验设计及响应面法筛选适于植物乳杆菌YSQ株大规模发酵的低成本培养基。结果表明:单因素试验确定培养基成分:碳源为玉米粉、次粉+蔗糖;氮源为豆粕;番茄汁作为生长因子供体。利用Plackett-Burman试验设计筛选出影响植物乳杆菌生长的4个主要因子:次粉、豆粕、K2HPO4和MnSO4。最陡爬坡、中心优化组合及响应面分析确定培养基各组分的最适添加量为:玉米粉10g/L、豆粕15.5g/L、次粉1.2g/L、蔗糖5g/L、番茄汁150mL/L、K2HPO4 1.1g/L、MnSO4 0.28g/L、MgSO4 0.3g/L。优化后,植物乳杆菌发酵18h的菌落总数从优化前的8.73×108CFU/mL(玉米粉、豆粕、次粉的混合料)提高到2.68×1010CFU/mL。

关键词: 植物乳杆菌YSQ株, 培养基优化, Plackett-Burman设计, 响应面法

Abstract: One-factor-at-a-time method, Plackett-Burman design and response surface methodology were employed combinedly to optimize a growth medium based on low-cost agricultural by-products, like soybean meal, corn flour, wheat middlings and tomato juice for large-scale cultivation of Lactobacillus plantarum YSQ. The results showed that: 1) According to the results of one-factor-at-a-time experiments, in the culture medium, the carbon source were composed of corn flour, wheat middlings, the nitrogen source was soybean meal, and tomato juice was added as a growth factor. 2) As identified by Plackett-Burman design, the main factor that affect the growth of Lactobacillus plantarum YSQ were wheat middlings, soybean meal, K2HPO4 and MnSO4. 3) Collectively, the results of steepest ascent tests, central composition design and response surface analysis indicated that the optimal growth medium was composed of corn flour 10 g/L, soybean meal 15.5 g/L, bran 1.2 g/L, sucrose 5 g/L, tomato juice 150 mL/L, K2HPO4 1.1 g/L, MnSO4 0.28 g/L, MgSO4 0.3 g/L. The total viable count was 2.68 × 1010 CFU/mL after 18 h of culture in the optimized medium, while that obtained with a medium composed of a mixture of corn flour, soybean meal and bran was only 8.73 × 108 CFU/mL.

Key words: Lactobacillus plantarum YSQ, fermentation medium optimization, Plackett-Burman design, response surface methodology

中图分类号: