真空冷冻干燥与喷雾干燥长双歧杆菌的工艺比较研究

doi:10.7506/spkx1002-6630-201207040

食品科学 ›› 2012, Vol. 33 ›› Issue (7): 188-192.doi: 10.7506/spkx1002-6630-201207040

真空冷冻干燥与喷雾干燥长双歧杆菌的工艺比较研究

付博1，马齐1，王卫卫2，黄炯力3

1.陕西省科学院酶工程研究所，陕西省酶工程技术中心，陕西省酿造发酵产品质量监督检验站 2.西北大学生命科学学院 3.西安计量技术研究院

出版日期:2012-04-15 发布日期:2012-04-20
基金资助:
陕西省科学院青年基金资助项目(2010k-26)

Comparison of Vacuum Freeze-Drying and Spray-Drying Processes for Active Bifidobacterium longum Powder Production

FU Bo1，MA Qi1，WANG Wei-wei2，HUANG Jiong-li3

(1. Enzyme Engineering Technology Center of Shaanxi, Fermentation Product Quality Supervision and Inspection Station of Shaanxi, Enzyme Engineering Institute of Shaanxi Academy of Sciences, Xi’an 710600, China；2. College of Life Science, Northwest University, Xi’an 710069, China；3. Xi’an Institute of Measurement Technology, Xi’an 710068, China)

Online:2012-04-15 Published:2012-04-20

摘要/Abstract

摘要： 为有效延长双歧杆菌的保存期和耐受性，利用真空冷冻干燥及喷雾干燥法制备活性菌粉。研究两种干燥方法的工艺参数，并对干燥得到的活性菌粉性能进行比较。真空冷冻干燥法制备的长双歧杆菌活性菌粉的活菌数为7.98×109CFU/g、存活率90.9%、含水量3.8%，4℃保藏90d后活菌数为2.5×108CFU/g，菌粉的水溶性好；喷雾干燥法制备的活性菌粉的活菌数为4.4×109CFU/g、存活率82.2%、包埋率71.9%、含水量5.6%，4℃保藏90d后活菌数为4×108CFU/g，菌粉在水中的溶解时间较长。真空冷冻干燥成本较高、操作时间长、菌粉的保藏期较短；而喷雾干燥工艺简单、生产成本低、工作效率高，包埋的菌粉保藏期较长。

关键词: 真空冷冻干燥, 喷雾干燥, 长双歧杆菌, 活性菌粉, 工艺, 比较

Abstract: For long term storage and to improve its stress resistance, Bifidobacterium longum was made into active bacteria powder by vacuum freeze-drying or spray-drying. In this study, we first optimized the technological conditions for active Bifidobacterium longum powder production and then compared these two processes in term of bacterial survival rate, cost, and ease of operation. The viable count, survival rate, moisture and viable count after storage for 90 days at 4 ℃ of vacuum freeze-dried active Bifidobacterium longum powder were 7.98 × 109 CFU/g, 90.9%, 3.8%, and 2.5 × 108 CFU/g, respectively. The powder dissolved easily in water. In contrast, the viable count, survival rate, encapsulation efficiency, moisture and viable count after storage for 90 days at 4 ℃ of spray-dried Bifidobacterium longum powder were 4.4 × 109 CFU/g, 82.2%, 71.9%, 5.6% and 4 × 108 CFU/g, respectively. It took longer time to dissolve in water when compared to vacuum-freeze dried powder. Vacuum freeze-drying was more complicated to operate, time-consuming and expensive than spray-drying. In conclusion, these two processes have both their advantages and disadvantages, which make them fit for different purposes.

Key words: vacuum freeze-drying, spray-drying, Bifidobacterium longum, active bacteria powder, process, comparison

中图分类号:

Q939.9

付博，马齐，王卫卫，黄炯力. 真空冷冻干燥与喷雾干燥长双歧杆菌的工艺比较研究[J]. 食品科学, 2012, 33(7): 188-192.

FUBo，MAQi，WANGWei-wei，HUANGJiong-li. Comparison of Vacuum Freeze-Drying and Spray-Drying Processes for Active Bifidobacterium longum Powder Production[J]. FOOD SCIENCE, 2012, 33(7): 188-192.

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真空冷冻干燥与喷雾干燥长双歧杆菌的工艺比较研究

Comparison of Vacuum Freeze-Drying and Spray-Drying Processes for Active Bifidobacterium longum Powder Production

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