食品科学 ›› 2012, Vol. 33 ›› Issue (11): 247-251.doi: 10.7506/spkx1002-6630-201211052

• 生物工程 • 上一篇    下一篇

红曲菌的复合诱变及其固态发酵条件的优化

杨欢欢,胡中泽*   

  1. 武汉工业学院食品科学与工程学院
  • 出版日期:2012-06-15 发布日期:2012-07-27
  • 基金资助:
    湖北省自然科学基金重点项目(2009CDA099)

Combined Mutation of Monascus and Optimization of Fermentation Conditions for Monacolin K Production

YANG Huan-huan,HU Zhong-ze*   

  1. (College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China)
  • Online:2012-06-15 Published:2012-07-27

摘要: 通过紫外线和LiCl对一株红曲菌MP进行复合诱变,并通过响应面法对其固态发酵条件进行优化分析。结果表明:在前期温度为30℃,后期温度为24℃,初始含水量为60%的条件下,Monacolin K的产量最高可达5.33mg/g。

关键词: 红曲菌, 复合诱变, Monacolin K, 响应面

Abstract: A strain of Monascus purpureu was treated with UV irradiation and its spore was then incubated in media containing LiCl to induce mutation. A mutant strain with high yield of monacolin K was obtained and its fermentation conditions were optimized for monacolin K production. The results showed that the optimal fermentation conditions were initial temperature of 30 ℃, late temperature of 24 ℃ and original moisture content of germinated brown rice of 60%. Under these conditions, the yield of monacolin K reached 5.33 mg/g.

Key words: Monascus, combined mutation, monacolin K, response surface methodology

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