食品科学 ›› 2012, Vol. 33 ›› Issue (12): 83-88.doi: 10.7506/spkx1002-6630-201212016

• 工艺技术 • 上一篇    下一篇

响应面法优化油菜蜂花粉核苷类成分提取工艺

孙丽萍1,黄 兰1,2,徐 响1,庞 杰2,何 伟1,2,穆雪峰1,2,沈新锋1,2   

  1. 1.中国农业科学院蜜蜂研究所 2.福建农林大学食品科学学院
  • 出版日期:2012-06-25 发布日期:2012-07-27
  • 基金资助:
    “十二五”国家科技支撑计划项目(2011BAD33B04); 国家现代农业(蜂)产业技术体系建设专项(CARS-45KXJ18);科技部农业科技成果转化资金项目(2009GB23260456)

Optimization of Nucleosides Extraction from Rape Bee Pollen Using Response Surface Methodology

SUN Li-ping1,HUANG Lan1,2,XU Xiang1,PANG Jie2,HE Wei1,2,MU Xue-feng1,2,SHEN Xin-feng1,2   

  1. (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Online:2012-06-25 Published:2012-07-27

摘要: 目的:考察油菜蜂花粉核苷类成分的提取条件,确定最佳提取工艺。方法:先通过单因素试验获得油菜蜂花粉核苷类成分提取时间、提取温度及料液比的优化范围,再利用Box-Behnken试验设计原理和3因素3水平的响应面分析法优化蜂花粉核苷类成分的提取工艺。结果:3因素的优化范围分别为40~60min、45~65℃、1:60~1:80(g/mL)。结论:最终优化条件为提取时间53min、温度60℃、料液比1:70(g/mL),该条件下,油菜蜂花粉中核苷类成分总提取量为(705.2±0.006)mg/100g。

关键词: 响应面法, 核苷类成分, 油菜蜂花粉, Box-Behnken, 超声波提取, 优化

Abstract: Nucleosides extraction from rape bee pollen was optimized using response surface methodology. The appropriate ranges of extraction time, extraction temperature and material-to-liquid ratio were obtained by one-factor-at-a-time experiments for subsequent optimization using response surface analysis based on a three-variable, three-level central composite design. The optimal ranges of extraction time, extraction temperature and material-to-liquid ratio were 40-60 min, 45-65 ℃ and 1:60-1:80 (g/mL), respectively. The optimized extraction conditions were 53 min, 60 ℃, and 1:70 (g/mL). Under these conditions, the extraction rate of nucleosides from rape bee pollen was (705.2 ±0.006) mg/100 g.

Key words: response surface methodology, nucleosides, rape bee pollen, Box-Behnken design, ultrasonic extraction, optimization

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