食品科学 ›› 2012, Vol. 33 ›› Issue (13): 26-30.doi: 10.7506/spkx1002-6630-201213003

• 基础研究 • 上一篇    下一篇

体内外消化高直链抗性水稻淀粉的波谱特性

满建民,周兴华,董英,刘巧泉,韦存虚   

  1. 1.扬州大学 教育部植物功能基因组学重点实验室,江苏省作物遗传生理重点实验室 2.江苏大学食品与生物工程学院
  • 出版日期:2012-07-15 发布日期:2012-07-27
  • 基金资助:
    农业部转基因生物新品种培育科技重大专项(2009ZX-08011-003B);国家自然科学基金面上项目(31071342); 江苏省作物学优势学科资助项目

Spectroscopic Properties of in vivo and in vitro Digestive Resistant Starch from High-Amylose Transgenic Rice

MANJian-min,ZHOUXing-hua,DONGYing,LIUQiao-quan,WEICun-xu   

  1. (1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
  • Online:2012-07-15 Published:2012-07-27

摘要: 以基因工程培育出来的高直链抗性淀粉水稻(TRS)为材料,利用粉末X射线衍射、傅里叶变换红外光谱分析技术,研究体内外消化TRS淀粉的波谱特性。结果表明:不被消化的抗性淀粉直链淀粉含量显著升高;TRS淀粉为C型晶体淀粉,由A型和B型晶体淀粉组成,其中A型晶体比B型晶体消化降解快,B型晶体具有抗消化降解的作用;TRS淀粉的无定形区域易被消化降解。

关键词: 水稻, 高直链淀粉, 体内消化, 体外消化, 波谱特性

Abstract: In this study, the spectroscopic properties of in vivo and in vitro digestive resistant starches from a high-amylose transgenic rice line (TRS) established through antisense RNA inhibition of starch branching enzymes were investigated by X-ray powder diffraction and Fourier transform infrared spectroscopy. The results showed that the amylose contents of non-digestive TRS resistant starch significantly increased. TRS starch was C-type crystalline starch, a mixture of A- and B-type crystalline starches. The degradation of the A-type crystalline starch was faster than that of B-type starch, and the B-type crystalline starch showed a high resistance to digestion. The amorphous starch of starch granule was easy to be degraded. These data could be useful for various applications of high-amylose crop in the food and nonfood industry.

Key words: rice, high-amylose starch, in vivo digestion, in vitro digestion, spectroscopic property

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