紫外和He-Ne 激光复合诱变获得高产γ - 聚谷氨酸产生菌的研究

doi:10.7506/spkx1002-6630-201213039

食品科学 ›› 2012, Vol. 33 ›› Issue (13): 183-186.doi: 10.7506/spkx1002-6630-201213039

紫外和He-Ne 激光复合诱变获得高产γ - 聚谷氨酸产生菌的研究

乔长晟，李雪，兰铃方，陈笑，马正旺，李政

1.天津市工业微生物重点实验室，工业发酵微生物教育部重点实验室，天津科技大学生物工程学院 2.天津北洋百川生物技术有限公司 3.天津工业大学纺织学院

出版日期:2012-07-15 发布日期:2012-07-27
基金资助:
天津市科技支撑重点计划项目(09ZCKFSH00800)

Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser

QIAOChang-sheng，LIXue，LANLing-fang，CHENXiao，MAZheng-wang，LIZheng

(1. Tianjin Key Laboratory of Industry Microbiology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China；2. Tianjin Peiyang Biotrans Biotech Co. Ltd., Tianjin 300457, China；3. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China)

Online:2012-07-15 Published:2012-07-27

摘要/Abstract

摘要： 以地衣芽孢杆菌(Bacillus licheniformis)BCPG006为出发菌株，通过紫外线和He-Ne激光复合诱变处理，后将诱变菌悬液涂布到抗性平板，选育出一株高产γ-聚谷氨酸的突变株BCPG078，经过摇瓶发酵，培养温度37℃、初始pH7、摇床转速220r/min、培养周期72h，测得γ-聚谷氨酸的产量为16g/L，为出发菌γ-聚谷氨酸的产量(6g/L)的2.67倍。传代实验证明，该突变株遗传性能稳定。说明紫外线和He-Ne激光复合诱变是γ-聚谷氨酸产生菌地衣芽孢杆菌有效的选育方法。

关键词: γ-聚谷氨酸, 复合诱变, 抗性平板, 发酵条件

Abstract: Bacillus licheniformis BCPG006 was mutagenized by means of both UV and He-Ne laser. A mutant strain named as Bacillus licheniformis BCPG078 having a high ability to produce γ-polyglutamic acid (γ-PGA), was obtained by resistance screening. The γ-PGA yield of the mutant strain was 16 g/L during 72 h of shake flask culture at 37 ℃, initial pH 7 and 200 r/min, which was 2.67-fold higher than that obtained from the original strain (6 g/L). These results suggest that the mutant strain has strong genetic stability. Meanwhile, combination of UV and He-Ne laser provides an effective method for the screening of γ-PGA-producing strains.

Key words: γ -PGA, compound mutation, resistant plate, fermentation condition

中图分类号:

Q815

乔长晟，李雪，兰铃方，陈笑，马正旺，李政. 紫外和He-Ne 激光复合诱变获得高产γ - 聚谷氨酸产生菌的研究[J]. 食品科学, 2012, 33(13): 183-186.

QIAOChang-sheng，LIXue，LANLing-fang，CHENXiao，MAZheng-wang，LIZheng. Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser[J]. FOOD SCIENCE, 2012, 33(13): 183-186.

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紫外和He-Ne 激光复合诱变获得高产γ - 聚谷氨酸产生菌的研究

Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser

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