食品科学 ›› 2012, Vol. 33 ›› Issue (13): 217-220.doi: 10.7506/spkx1002-6630-201213046

• 生物工程 • 上一篇    下一篇

紫外- 氯化锂复合诱变选育中性植酸酶高产菌株

王陶,李文,尹龙,刘洋   

  1. 徐州工程学院食品(生物)工程学院
  • 出版日期:2012-07-15 发布日期:2012-07-27
  • 基金资助:
    江苏省高校自然科学基础研究面上项目(10KJD180006); 江苏省科技创新与成果转化(重大科技支撑与自主创新)专项(BE2011644); 徐州工程学院科研基金培育课题(XKY2010112)

Mutation Breeding of Neutral Phytase-Producing Strain Using Both UV Light and Lithium Chloride

WANGTao,LIWen,YINLong,LIUYang   

  1. (College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China)
  • Online:2012-07-15 Published:2012-07-27

摘要: 以放射型根瘤杆菌为出发菌株,对其进行紫外-氯化锂(LiCl)复合诱变筛选,得到一株产中性植酸酶酶活力较高的菌株。当培养温度为30℃、pH值为7.0、接种量为10%时,经96h培养后,该菌株产中性植酸酶活力最高达到18.49U/mL,比原始菌株提高47.68%,且发酵周期缩短12h,该菌株具有良好的遗传稳定性。

关键词: 中性植酸酶, 紫外- LiCl复合诱变, 酶活力, 稳定性

Abstract: Agrobacterium radiobacter AR01 was mutagenized using ultraviolet (UV) irradiation and subsequent lithium chloride treatment to obtain a high-yield neutral phytase-producing strain. The neutral phytase activity of the strain reached up to 18.49 U/mL after 96 h cultivation at pH 7.0, 30 ℃ and an inoculum amount of 10%, which was 47.68% higher than that of the original strain. The fermentation cycle was shortened by 12 h. Moreover, the mutant strain showed good hereditary stability.

Key words: neutral phytase, mutation induced by both UV and lithium chloride, enzymatic activity, stability

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