葛根素对运动疲劳大鼠海马NO-cGMP
信号系统的影响

doi:10.7506/spkx1002-6630-201213059

食品科学 ›› 2012, Vol. 33 ›› Issue (13): 276-279.doi: 10.7506/spkx1002-6630-201213059

葛根素对运动疲劳大鼠海马NO-cGMP 信号系统的影响

郭雅，习雪峰

1.西北工业大学体育部 2.河南大学体育学院

收稿日期:2020-04-01 修回日期:2020-04-01 出版日期:2012-07-15 发布日期:2012-07-27

Influence of Puerarin Administration on Hippocampal NO-cGMP Signaling System in Rats with Exercise-Induced Fatigue

GUOYa，XIXue-feng

(1. Physical Education Department, Northwest Polytechnical University, Xi’an 710072, China； 2. College of Physical Education, Henan University, Kaifeng 475001, China)

Received:2020-04-01 Revised:2020-04-01 Online:2012-07-15 Published:2012-07-27

摘要/Abstract

摘要： 目的：研究葛根素对运动疲劳大鼠认知能力和海马组织NO-cGMP信号系统的影响。方法：选取成年雄性SD大鼠30只。将大鼠随机分为3组：安静对照组、运动对照组、运动加药组，每组10只大鼠。对大鼠进行为期10d递增负荷游泳，测试大鼠认知能力和海马iNOS mRNA、nNOS mRNA表达和cGMP、NO含量。结果：运动对照组大鼠中心区停留时间百分比、通过原平台位置次数和nNOS mRNA含量较安静对照组显著下降，大鼠海马NO含量、cGMP含量、iNOS mRNA表达显著增加；运动加药组大鼠中心区停留时间百分比、通过原平台位置次数和nNOS mRNA表达较运动对照组显著增加。大鼠海马组织NO含量、iNOS mRNA表达、cGMP含量较运动对照组显著降低。结论：葛根素能显著降低运动疲劳大鼠海马组织的NO含量，其结果可能与iNOS mRNA表达的降低有关，并可能通过降低海马组织NO含量，使运动疲劳大鼠海马cGMP含量趋于稳定以实现运动疲劳大鼠的神经保护功能。

关键词: 葛根素, 海马, 运动疲劳, NO-cGMP信号系统

Abstract: Objective: To investigate the effect of puerarin on cognitive ability and hippocampal tissue NO-cGMP signaling in rats with exercise induced fatigue. Methods: A total of 30 adult male SD rats were randomly assigned to 3 groups (10 rats in each group): no exercise control, exercise and exercise plus puerarin groups. The rats in both exercise groups were subjected to increasing weight-loading swimming training for 10 d. The cognitive ability of rats was evaluated by morris water maze test, and the hippocampal iNOS mRNA and nNOS mRNA expression levels and cGMP and NO contents were measured. Results: The exercise control group showed a significant decrease in percent residence time in the central area, the number of original platform passes, and hippocampal nNOS mRNA expression level but a significant increase in hippocampal cGMP and NO contents and iNOS mRNA expression level as compared to the no exercise control. The percent residence time in the central area, the number of original platform passes and nNOS mRNA expression level of the exercise plus puerarin group were significantly higher than those of the exercise control group, whereas the hippocampal cGMP and NO contents and iNOS mRNA expression lelvel of the exercise plus puerarin group were significantly lower than those of the exercise control group. Conclusion: Puerarin administration can result in a marked decrease in hippocampal NO content in rats with exercise induced fatigue, which may be related to the decreased expression of iNOS mRNA. Moreover, puerarin has some neuroprotective effect on rats with exercise induced fatigue by inhibiting hippocampal NO and stabilizing cGMP production.

Key words: puerarin, hippocampus, exercise induced fatigue, NO-cGMP signaling system

中图分类号:

TS201.4

郭雅，习雪峰. 葛根素对运动疲劳大鼠海马NO-cGMP 信号系统的影响[J]. 食品科学, 2012, 33(13): 276-279.

GUOYa，XIXue-feng. Influence of Puerarin Administration on Hippocampal NO-cGMP Signaling System in Rats with Exercise-Induced Fatigue[J]. FOOD SCIENCE, 2012, 33(13): 276-279.

[1]	高华武，王艳，周鹏，叶树，宋航，汪光云，蔡标. 金雀异黄酮通过调控Ca2＋-CaMKIV通路对Aβ25-35诱导海马神经元损伤的保护作用[J]. 食品科学, 2021, 42(13): 121-126.
[2]	陈慧，马璇，曹丽行，赵冲，尹淑涛，张秋，扈洪波. 运动疲劳机制及食源性抗疲劳活性成分研究进展[J]. 食品科学, 2020, 41(11): 247-258.
[3]	闫珍珍，张东，林听听，刘鑫，肖冬雪，郭全友. 雌雄灰海马和三斑海马营养价值与功能性成分对比分析[J]. 食品科学, 2019, 40(16): 206-212.
[4]	罗程印，程远渡，易有金，夏菠，周红丽，曹熙. 植物甾醇酯和葛根素复合微胶囊的制备工艺优化[J]. 食品科学, 2016, 37(6): 26-33.
[5]	魏述永. 葛根及葛根素脑保护作用的研究进展[J]. 食品科学, 2015, 36(17): 259-263.
[6]	程远渡1，易有金1,*，易传祝2，夏菠1，夏延斌1. 植物甾醇酯与葛根素对营养肥胖小鼠的减肥功效[J]. 食品科学, 2015, 36(13): 223-228.
[7]	许苗苗1，王素素1，李辉1,2,*，黄宇琳1，逯翠梅1，蒋剑波1. 沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉[J]. 食品科学, 2015, 36(10): 11-15.
[8]	邱远，芮昕，谢翌冬，李伟，陈晓红，姜梅，董明盛*. 响应面法优化超声提取葛根素工艺[J]. 食品科学, 2014, 35(6): 1-5.
[9]	孙涛，李铭，谢晶，薛斌，李立. 槲皮素与葛根素对食品体系中非酶糖基化的抑制作用[J]. 食品科学, 2014, 35(3): 47-49.
[10]	朱振元，薛婧，刘晓翠，孟梦，唐亚丽，张军，郭蓉. 葛根素及葛根异黄酮对小鼠急性醉酒预防和解酒效果的研究[J]. 食品科学, 2014, 35(15): 247-250.
[11]	叶惠煊，李芝，李小军，黄玮超，谢霞，刘向前*. 湘葛一号茎叶中5 种异黄酮成分的高效液相色谱法定量分析[J]. 食品科学, 2014, 35(14): 141-144.
[12]	池爱平，李虹，康琛喆，郭欢欢，张海猛，郭飞，王一民. 富硒茶多糖的提取及其对运动疲劳恢复的影响[J]. 食品科学, 2014, 35(13): 240-244.
[13]	李适，岳明珠，李银花，傅冬和. 正交试验优化葛根素的大孔树脂纯化工艺[J]. 食品科学, 2013, 34(16): 89-92.
[14]	张海晖1，武妍1，张瑞1，张爽1，段玉清1,*，孙桂波2，孙晓波2. 莲房原花青素对极低频电磁场致海马神经元损伤的预防作用[J]. 食品科学, 2013, 34(13): 229-233.
[15]	仝海娟1，李小燕1,2,*，黄春梅1，雷福厚1,2，李鹏飞1,2，谭学才1,2. 马来松香乙二醇丙烯酸酯-丙烯酰胺共聚物对葛根素的吸附性能[J]. 食品科学, 2013, 34(1): 27-32.

葛根素对运动疲劳大鼠海马NO-cGMP 信号系统的影响

Influence of Puerarin Administration on Hippocampal NO-cGMP Signaling System in Rats with Exercise-Induced Fatigue

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价