毛蕊花糖对巨噬细胞RAW264.7免疫调节作用的研究

doi:10.7506/spkx1002-6630-201307052

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 250-253.doi: 10.7506/spkx1002-6630-201307052

毛蕊花糖对巨噬细胞RAW264.7免疫调节作用的研究

苏娣，张芸，戴竹青，叶红，胡冰，曾晓雄*

南京农业大学食品科技学院，江苏南京 210095

收稿日期:2012-07-21 修回日期:2013-02-26 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 曾晓雄 E-mail:zengxx2002@yahoo.com.cn
基金资助:
国家自然科学基金项目(31171750)；江苏高校优势学科建设工程资助项目

Immunoregulatory Function of Verbascose on Macrophage Cell Line RAW264.7

SU Di，ZHANG Yun，DAI Zhu-qing，YE Hong，HU Bing，ZENG Xiao-xiong*

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2012-07-21 Revised:2013-02-26 Online:2013-04-15 Published:2013-03-20
Contact: ZENG Xiao-xiong E-mail:zengxx2002@yahoo.com.cn

摘要/Abstract

摘要： 目的：明确毛蕊花糖对巨噬细胞RAW264.7免疫功能的调节作用。方法：分别以质量浓度25、50、100、200、400μg/mL的毛蕊花糖对RAW264.7巨噬细胞进行培养，以10μg/mL的脂多糖(LPS)为阳性对照组、不加任何样品的培养基为空白对照组。通过噻唑蓝(MTT)实验、吞噬中性红实验、NO释放量测定及酶联免疫吸附法测定细胞因子(IL-6、IL-1β、IFN-α、IFN-γ)的分泌量，评价毛蕊花糖的免疫调节作用。结果：质量浓度为25～400μg/mL的毛蕊花糖对巨噬细胞RAW264.7作用24h后，可显著提高巨噬细胞的增殖活性、吞噬活性、NO释放量以及细胞因子的分泌水平，并且毛蕊花糖溶液质量浓度为200μg/mL时效果最好。结论：毛蕊花糖对小鼠腹腔巨噬细胞系RAW264.7具有免疫调节活性，是一种良好的免疫增强剂。

关键词: 毛蕊花糖, 巨噬细胞RAW264.7, 免疫调节活性

Abstract: Objective: To investigate the immunoregulatory function of verbascose on macrophage cell line RAW264.7. Methods: RAW264.7 cells were cultured in a 96-well plate with different concentrations of verbascose (25, 50, 100, 200 μg/mL and 400 μg/mL), and the role of verbascose was evaluated based on the proliferative activity of cultured splenocytes as determined by MTT assay, nitric oxide (NO) production and cytokines (IL-6, IL-1β, IFN-α and IFN-γ) expression levels, neutral red phagocytosis. In the present study, lipopolysaccharide (LPS, 10 μg/mL) was used as a positive control, and treatment without verbascose or LPS was used as a negative control. Results: Verbascose significantly improved the proliferation, phagocytosis, NO level and cytokines expression levels when RAW264.7 cells were cultured for 24 h in the studied concentration range. Verbascose at a dose of 200 μg/mL showed the highest effect. Conclusion: Verbascose has an immunomodulatory effect on macrophage cell line RAW264.7 as a potential immunomodulatory enhancer.

Key words: verbascose, macrophage cell RAW264.7, immunostimulatory activity

中图分类号:

TS201.4

苏娣，张芸，戴竹青，叶红，胡冰，曾晓雄*. 毛蕊花糖对巨噬细胞RAW264.7免疫调节作用的研究[J]. 食品科学, 2013, 34(7): 250-253.

SU Di，ZHANG Yun，DAI Zhu-qing，YE Hong，HU Bing，ZENG Xiao-xiong*. Immunoregulatory Function of Verbascose on Macrophage Cell Line RAW264.7[J]. FOOD SCIENCE, 2013, 34(7): 250-253.

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毛蕊花糖对巨噬细胞RAW264.7免疫调节作用的研究

Immunoregulatory Function of Verbascose on Macrophage Cell Line RAW264.7

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