葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响

doi:10.7506/spkx1002-6630-201307051

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 245-249.doi: 10.7506/spkx1002-6630-201307051

葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响

李宇杰，张召锋，戴小倩，丁叶，马小陶，姜燕飞，鲍雷，李勇*

北京大学公共卫生学院营养与食品卫生学系，北京 100191

收稿日期:2012-01-15 修回日期:2013-03-01 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 李勇 E-mail:liyong@bjmu.edu.cn
基金资助:
国家自然科学基金项目(81072293)

Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats

LI Yu-jie，ZHANG Zhao-feng，DAI Xiao-qian，DING Ye，MA Xiao-tao，JIANG Yan-fei，BAO Lei，LI Yong*

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China

Received:2012-01-15 Revised:2013-03-01 Online:2013-04-15 Published:2013-03-20
Contact: LI Yong E-mail:liyong@bjmu.edu.cn

摘要/Abstract

摘要： 目的：观察葡萄籽原花青素(GSPE)对Ⅱ型糖尿病大鼠视网膜病变的影响。方法：雄性SD大鼠高脂饲料喂养4周后，腹腔注射链脲佐菌素(STZ)造成糖尿病模型，成模后随机分为糖尿病模型组和原花青素低、中、高剂量组；以基础饲料喂养为正常对照组。原花青素低、中、高剂量组分别为125、250、500mg/(kg·d)(以体质量计)，通过每天灌胃水溶液的方式进行干预，糖尿病模型组和正常对照组每天以蒸馏水灌胃。连续饲养16周后，测定其体质量、空腹血糖、血清糖基化终末产物(AGEs)、细胞间黏附分子(ICAM-1)、血管间黏附分子(VCAM-1)，镜下观察视网膜的变化，免疫组化法测定视网膜中糖基化终产物受体(RAGE)的表达。结果：GSPE干预组血糖较糖尿病模型组有一定下降，但无显著性差异(P＞0.05)；GSPE高剂量组可以有效降低血清中AGEs水平(P＜0.05)；GSPE各干预组可以有效降低血清中ICAM-1水平(P＜0.05)；GSPE低剂量和高剂量组可以有效降低血清中VCAM-1水平(P＜0.05)；从组织学上，GSPE可以减轻视网膜损伤，保持视网膜各层完整；GSPE能降低RAGE在视网膜中的表达。结论：葡萄籽原花青素可以有效改善Ⅱ型糖尿病大鼠视网膜病变。

关键词: 葡萄籽原花青素, 糖尿病视网膜病变, 糖基化终末产物, 炎症因子

Abstract: Objective: To investigate the effect of grape seed proanthocyanidin extract (GSPE) on type Ⅱ diabetic retinopathy in rats. Methods: To establish type Ⅱ diabetic rat models, male SD rats fed high fat diet for 4 weeks were injected with streptozotocin (STZ, 0.01 mol/L, 30 mg/(kg?d)). The diabetic rats were randomly divided into diabetes-vehicle group (DM control group), three GSPE-treated groups. Normal rats were enrolled as normal control group. The low, medium and high-dose GSPE-treated groups were administrated at 125, 250 mg/(kg?d) and 500 mg/(kg?d), respectively. DM control group and normal control group were given equal volume of distilled water. After 16 weeks, body weight, fasting plasma glucose (FPG), advanced glycation endproducts (AGEs), ICAM-1 and VCAM-1 were measured. HE stain retina paraffin section was examined under a microscope. Receptor for RAGEs was detected by SP immunohistochemical method. Results: There was no difference (P ＞ 0.05) between DM control group and GSPE-treated groups. Compared with DM control group, AGEs in GSPE high dose group were decreased (P ＜ 0.05), ICAM-1 (all GSPE-treated groups) and VCAM-1 (GSPE low and high dose groups) were decreased (P ＜ 0.05). The retina of GSPE-treated groups had fewer damaged areas and lower RAGE expression in histology. Conclusion: GSPE has a positive effect on type Ⅱ diabetic retinopathy in rats.

Key words: grape seed proanthocyanidin extract (GSPE), diabetic retinopathy (DR), advanced glycation endproducts (AGEs), inflammatory factor

中图分类号:

151.2

李宇杰，张召锋，戴小倩，丁叶，马小陶，姜燕飞，鲍雷，李勇*. 葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响[J]. 食品科学, 2013, 34(7): 245-249.

LI Yu-jie，ZHANG Zhao-feng，DAI Xiao-qian，DING Ye，MA Xiao-tao，JIANG Yan-fei，BAO Lei，LI Yong*. Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats[J]. FOOD SCIENCE, 2013, 34(7): 245-249.

参考文献

[1]Bhaduri G, Bandyopadhyay M.Challenges to confront diabetic retinopathy and retinopathy of prematurity[J].Journal of the Indian Medical Association,2005,103(7):363-
[2]Bagchi D, Bagchi M, Stohs SJ, et al.Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention[J].Toxicology,2000,148(2-3):187-197
[3]Pinent M, Blay M, Blade M, Salvado M, Arola L, Ardevol A.Grape seed-derived procyanidins have an antihyperglycemic effect in streptozotocin-induced diabetic rats and insulinomimetic activity in insulin-sensitive cell lines[J].Endocrinology,2004,145(11):4985-
[4]Van Hecke M, Dekker J, Nijpels G, et al.Inflammation and endothelial dysfunction are associated with retinopathy: the Hoorn Study[J].Diabetologia,2005,48(7):1300-1306
[5]周雁, 马亚兵, 高海青, 程梅, 由倍安.葡萄籽多酚抗糖尿病大鼠非酶糖基化实验研究[J].中华老年医学杂志,2005,24(1):49-52
[6]Sajithlal G, Chithra P, Chandrakasan G.The role of metal-catalyzed oxidation in the formation of advanced glycation end products: an in vitro study on collagen[J].Free Radical Biology and Medicine,1998,25(3):265-269
[7]Mohr S.Potential new strategies to prevent the development of diabetic retinopathy[J].Expert Opinion on Investigational Drugs,2004,13(3):189-198
[8]Ben-Mahmud BM, Mann GE, Datti A, Orlacchio A, Kohner EM, Chibber R.Tumor Necrosis Factor-α in Diabetic Plasma Increases the Activity of Core 2 GlcNAc-T and Adherence of Human Leukocytes to Retinal Endothelial Cells[J].Diabetes,2004,53(11):2968-
[9]Miyamoto K, Khosrof S, Bursell SE, et al.Prevention of leukostasis and vascular leakage in streptozotocin-induced diabetic retinopathy via intercellular adhesion molecule-1 inhibition[J].Proceedings of the National Academy of Sciences,1999,96(19):10836-
[10]Ma L, Gao H, Li B, Ma Y, You B, Zhang F.Grape Seed Proanthocyanidin Extracts Inhibit Vascular Cell Adhesion Molecule Expression Induced by Advanced Glycation End Products Through Activation of Peroxisome Proliferators-Activated Receptor γ[J].Journal of cardiovascular pharmacology,2007,49(5):293-
[11]Prior RL, Gu L.Occurrence and biological significance of proanthocyanidins in the American diet[J].Phytochemistry,2005,66(18):2264-2280

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葡萄籽原花青素对Ⅱ型糖尿病大鼠视网膜病变的影响

Effect of Grape Seed Proanthocyanidin Extract (GSPE) on Type Ⅱ Diabetic Retinopathy in Rats

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