非水胶束电动毛细管色谱在线富集测定果蔬中克百威和甲萘威残留

doi:10.7506/spkx1002-6630-201308037

食品科学 ›› 2013, Vol. 34 ›› Issue (8): 176-181.doi: 10.7506/spkx1002-6630-201308037

非水胶束电动毛细管色谱在线富集测定果蔬中克百威和甲萘威残留

童明珠，陈冠华*，武传芹，郭东山，方柔，张丽

江苏大学食品与生物工程学院，江苏镇江 212013

收稿日期:2011-12-19 修回日期:2013-03-18 出版日期:2013-04-25 发布日期:2013-05-07
通讯作者: 陈冠华 E-mail:chengh@ujs.edu.cn
基金资助:
教育部高等学校博士学科点专项基金;江苏大学科研基金

Determination of Carbofuran and Carbaryl Residues in Fruits and Vegetables by Nonaqueous Micellar Electrokinetic Capillary Chromatography with Online Enrichment

TONG Ming-zhu，CHEN Guan-hua*，WU Chuan-qin，GUO Dong-shan，FANG Rou，ZHANG Li

College of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China

Received:2011-12-19 Revised:2013-03-18 Online:2013-04-25 Published:2013-05-07
Contact: Guan-Hua CHEN E-mail:chengh@ujs.edu.cn

摘要/Abstract

摘要： 建立基于非水胶束电动毛细管色谱在线富集技术测定蔬果中克百威和甲萘威残留的新方法。对影响分离和富集效果的缓冲液参数、分离电压和进样时间进行优化。运行缓冲溶液为含100mmol/L十二烷基硫酸钠、30mmol/L乙酸钠的甲酰胺-乙腈(85:15，V/V)溶液，表观pH值为9.4；分离电压20kV；进样时间150s。在此优化条件下，克百威和甲萘威的富集倍数分别为678和758倍；检出限分别为31μg/L和7.9μg/L；6次重复测定的峰面积相对标准偏差为2.47%、1.76%；平均加标回收率为80.4%～96.2%和92.5%～104.1%。以上结果表明该方法具有良好的灵敏度和重现性，可用于果蔬中该两种农药残留的检测。

关键词: 非水胶束电动毛细管色谱, 克百威, 甲萘威, 甲酰胺, 富集

Abstract: A method of nonaqueous micellar electrokinetic capillary chromatography with online enrichment was developed to determine carbofuran and carbaryl residues in fruits and vegetables. The effects of buffer composition, apparent pH, applied voltage, and injection time were systematically discussed. The optimized buffer was composed of 100 mmol/L sodium dodecyl sulfate, 30 mmol/L sodium acetate, and formamide-acetonitrile (85:15, V/V), and its apparent pH was adjusted to 9.4. The applied voltage was 20 kV and the sample injection time was 150 s. Under these optimal conditions, peak enrichment factor was 678 and 758; detection limit was 31 μg/L and 7.9 μg/L; RSD was 2.47% and 1.76%; average recovery rates of spiked samples were 80.4%–96.2% and 92.5%–104.1% for carbofuran and carbaryl residues, respectively. Therefore, this method is of high sensitivity, good repeatability and can be used in the determination of both pesticide residues in fruits and vegetables.

Key words: nonaqueous micellar electrokinetic capillary chromatography；carbofuran；carbaryl residue, formamide, enrichment

中图分类号:

O657.8

童明珠，陈冠华*，武传芹，郭东山，方柔，张丽. 非水胶束电动毛细管色谱在线富集测定果蔬中克百威和甲萘威残留[J]. 食品科学, 2013, 34(8): 176-181.

TONG Ming-zhu，CHEN Guan-hua*，WU Chuan-qin，GUO Dong-shan，FANG Rou，ZHANG Li. Determination of Carbofuran and Carbaryl Residues in Fruits and Vegetables by Nonaqueous Micellar Electrokinetic Capillary Chromatography with Online Enrichment[J]. FOOD SCIENCE, 2013, 34(8): 176-181.

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非水胶束电动毛细管色谱在线富集测定果蔬中克百威和甲萘威残留

Determination of Carbofuran and Carbaryl Residues in Fruits and Vegetables by Nonaqueous Micellar Electrokinetic Capillary Chromatography with Online Enrichment

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