关键词: 甲基睾丸酮, 高效液相色谱法, 养殖水体, 底泥

Abstract:

A high performance liquid chromatography (HPLC) method has been established for the determination of
17α-methyltestosterone (17α-MeT) in water and sediment of tilapia ponds. 17α-MeT in water and sediment samples was
extracted using methylene chloride and methanol, respectively, followed by rotary evaporation. Then, the residues were
dissolved in methanol. Finally, 17α-MeT was detected using a UV detector and quantified by external standard method. The
average recovery rates of water spiked with 17α-MeT at 1.00–25.0 μg/L were 93.3%–101% with RSD of 3.0%–5.5%. The
average recovery rates of sediment spiked with 17α-MeT at 10–250 μg/kg were 88.7%–96.6% with RSD of 2.7%–6.2%. The
limits of detection for 17α-MeT in water and sediment were 0.5 μg/L and 5.0 μg/kg, respectively. The established method is
sensitive, accurate and suitable for the determination of 17α-MeT residues in aquaculture water and sediment samples．

Key words: 17&alpha, -methyltestosterone；high performance liquid chromatography；aquatic water；sediment