食品科学

• 生物工程 • 上一篇    下一篇

中药天麻成分对灰树花胞外多糖合成及相关关键酶的影响

贺宗毅,吴天祥,徐晓宝   

  1. 1.重庆市中药研究院,重庆 400065;2.贵州大学生命科学学院,贵州 贵阳 550025;3.贵州大学化学与化工学院,贵州 贵阳 550025
  • 出版日期:2013-06-15 发布日期:2013-06-03

Effect of Rhizoma Gastrodiae on Key Enenzyme Activities Involved in the Biosynthesis of Exopolysaccharides from Grifola frondosa in Submerged Culture

HE Zong-yi,WU Tian-xiang,XU Xiao-bao   

  1. 1. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;2. School of Life Sciences, Guizhou University,Guiyang 550025, China;3. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China
  • Online:2013-06-15 Published:2013-06-03

摘要:

采用液体深层发酵方式研究中药天麻对灰树花胞外多糖合成的影响,并用苯酚-硫酸法测定总糖含量。结果表明:天麻添加量为7g/L时能显著促进灰树花胞外多糖产量的增加。此时灰树花产胞外多糖产量平均为3.91g/L,与对照组的3.72g/L相比提高了5.1%。同时,对灰树花多糖合成代谢途径中的两个重要酶的酶活力进行测定。说明添加天麻成分发酵后,磷酸葡萄糖异构酶酶活力有所降低,磷酸葡萄糖变位酶酶活力则无明显变化。

关键词: 灰树花, 天麻, 胞外多糖, 磷酸葡萄糖异构酶, 磷酸葡萄糖变位酶

Abstract:

The effect of Rhizoma Gastrodiae on the biosynthesis of expolysaccharide (EPS) from Grifola frondosa in
submerged culture was studied. Total polysaccharide content was determined by phenol-sulphuric acid method. The results
showed that Rhizoma Gastrodiae at 7 g/L significantly promoted the biosynthesis of EPS in Grifola frondosa when compared
with blank control, increasing EPS yield from 3.72 g/L to 3.91 g/L. The measured activities of two key enzymes involved in
the synthesis of EPS indicated that after fermentation in the presence of Rhizoma Gastrodiae, PGI activity was decreased,
although α-PGM activity showed no obvious change.

Key words: Grifola frondosa;Rhizoma Gastrodiae;exopolysaccharide;PGI;&alpha, -PGM