食品科学

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低分子质量壳聚糖对镉胁迫引起河南华溪蟹氧化损伤的保护作用

樊冰心,任国锐,李瑞金,王 兰   

  1. 1.山西大学生命科学学院,山西 太原 030006;2.山西大学环境科学与工程研究中心,山西 太原 030006
  • 出版日期:2013-06-15 发布日期:2013-06-03

Protective Action of Low Molecular Weight Chitosan against Cadmium-induced Oxidative Damage in the Freshwater Crab Sinopotamon henanense

FAN Bing-xin,REN Guo-rui,LI Rui-jin,WANG Lan   

  1. 1. College of Life Science, Shanxi University, Taiyuan 030006, China;
    2. Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan 030006, China
  • Online:2013-06-15 Published:2013-06-03

摘要:

采用浸浴法,研究低分子质量壳聚糖(LMWC)对镉胁迫引起河南华溪蟹(Sinopotamon henanense)肝胰腺组织中镉积累情况、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量、谷胱甘肽硫转移酶(GST)活性和还原型谷胱甘肽(GSH)含量的影响,旨在评价LMWC的保护作用。设置镉处理组(29、58、87mg/L)、LMWC组(20、40、80mg/L)、LMWC组(20、40、80mg/L)和镉(58mg/L)联合处理组,对照组;处理时间为96h。结果显示:与对照组相比,LMWC单独作用下,SOD活性和MDA含量变化不显著,不会引起肝胰腺组织脂质过氧化。但58mg/L镉单独作用下,SOD活性显著降低,MDA含量显著增加,降低了机体抗氧化能力。镉积累量随着镉浓度升高逐渐增加,加入LMWC后镉积累量显著降低,且具有质量浓度-效应关系。GST活性和GSH含量随着镉质量浓度升高逐渐降低,而加入LMWC后其水平比镉染毒组(58mg/L)显著升高。LMWC对镉胁迫引起的肝胰腺组织氧化损伤可以起到良好的保护作用。

关键词: 河南华溪蟹, 低分子质量壳聚糖, 丙二醛, 超氧化物歧化酶, 谷胱甘肽硫转移酶, 还原型谷胱甘肽,

Abstract:

To investigate the protective effect of low molecular weight chitosan (LMWC) against cadmium-induced
toxicological activity, the freshwater crab Sinopotamon henanense was challenged with LMWC (20, 40 mg/L and 80 mg/L) and
or cadmium (29, 58 mg/L and 87 mg/L). Cadmium accumulation, superoxide dismutase (SOD) activity, malondialdehyde
(MDA) content, glutathione S-transferase (GST) activity and glutathione (GSH) content were measured in hepatopancreas
of S. henanense. The results showed that Cd2+ (58 mg/L) significantly decreased SOD activity and increased MDA
content in hepatopancreas of the crabs compared to the control group (P < 0.05), whereas LMWC did not change SOD
activity and MDA content in hepatopancreas. Also, there was an apparent accumulation of Cd2+ in hepatopancreas, which
was lessened in the presence of LMWC in a concentration-dependent manner. In addition, Cd2+ significantly decreased
the hepatopancreas GST activity and GSH content in the crabs relative to the control (P < 0.05). Co-treatment with
LMWC enhanced the GST activity and GSH content in hepatopancreas tissues compare with the crabs exposed to Cd2+
alone. According to these results, it can be concluded that LMWC has protective function against the adverse influence of
cadmium on Sinopotamon henanense.

Key words: Sinopotamon henanense, low molecular weight chitosan, malondialdehyde, superoxide dismutase, glutathione S-transferase, glutathione, cadmium