昆仑雪菊水溶性黄酮提取物对D-半乳糖致衰老小鼠学习记忆障碍的改善作用

doi:10.7506/spkx1002-6630-20180724-291

食品科学 ›› 2019, Vol. 40 ›› Issue (1): 162-168.doi: 10.7506/spkx1002-6630-20180724-291

昆仑雪菊水溶性黄酮提取物对D-半乳糖致衰老小鼠学习记忆障碍的改善作用

田勇1，周督2，邹双忆2，孙星宇2，郅琦1，李福香1，明建1,3,*

1.西南大学食品科学学院，重庆 400715；2.西南大学附属中学，重庆 400715；3.西南大学食品贮藏与物流研究中心，重庆 400715

出版日期:2019-01-15 发布日期:2019-01-22
基金资助:
中央高校基本科研业务费专项资金项目（XDJK2017B050）；重庆市中小学创新人才培养工程项目（CY180208）

Water Soluble Flavonoids from Coreopsis tinctoria Flowers Improve Learning and Memory Ability in D-Galactose-Induced Aging Mice

TIAN Yong1, ZHOU Du2, ZOU Shuangyi2, SUN Xingyu2, ZHI Qi1, LI Fuxiang1, MING Jian1,3,*

1. College of Food Science, Southwest University, Chongqing 400715, China; 2. High School Affiliated to Southwest University, Chongqing 400715, China; 3. Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, China

Online:2019-01-15 Published:2019-01-22

摘要/Abstract

摘要： 为探讨昆仑雪菊水溶性黄酮提取物（water soluble flavonoids from Coreopsis tinctoria flowers，CTWF）对D-半乳糖致衰老小鼠学习记忆障碍的影响，以SPF级昆明种小鼠为实验对象，随机分为正常组、衰老模型组、VE组（50 mg/（kg·d））和CTWF低、高剂量组（150、600 mg/（kg·d））。除正常组皮下注射生理盐水外，其余各组均注射D-半乳糖（300 mg/（kg·d）），连续42 d造模，注射1 h后，药物组分别灌胃VE和不同剂量CTWF，正常组和模型组灌胃等量生理盐水。采用Morris水迷宫测试各组小鼠学习记忆能力，并测定小鼠脑组织乙酰胆碱（acetylcholine，Ach）含量，乙酰胆碱酯酶（acetylcholinesterase，AchE）、超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力和丙二醛（malondialdehyde，MDA）含量。结果表明：CTWF能够显著提升衰老模型小鼠的学习记忆能力（P＜0.05），并显著提高脑组织SOD、GSH-Px活力和Ach含量（P＜0.05），降低AchE活力和MDA含量（P＜0.05）。因此，CTWF具有明显改善衰老模型小鼠学习记忆障碍的作用，其机制可能与提高脑组织胆碱能系统功能和减少自由基损伤有关。

关键词: 昆仑雪菊水溶性黄酮提取物, 学习记忆能力, 乙酰胆碱, 超氧化物歧化酶, 丙二醛

Abstract: Our aim in this study was to investigate the effects and mechanisms of water soluble flavonoids from Coreopsis tinctoria flowers (CTWF) on learning and memory ability in D-galactose-induced aging mice. Specific pathogen-free (SPF) Kunming mice were randomly divided into control, aging model, VE (50 mg/(kg·d)), and CTWF low- and high-dose groups (150 and 600 mg/(kg·d)). The mice in the control group were injected subcutaneously with normal saline while the mice in the other groups were successively injected with D-galactose (300 g/(kg·d)) for 42 days. At 1 hour after injection, the drug treatment groups were intragastrically administrated with VE and different dosage of CTWF while the mice from the control and model groups were administrated with an equal volume of normal saline. Learning and memory abilities were detected by Morris water maze test, and acetylcholinesterase (AchE), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities, and acetylcholine (Ach) and malondialdehyde (MDA) contents in brain tissues were measured. Results showed that CTWF significantly improved the learning and memory abilities of aging mice (P < 0.05), elevated SOD and GSH-Px activities and Ach content (P < 0.05), and reduced AchE activity and MDA content (P < 0.05). In conclusion, CTWF has a significant effect on improving D-galactose-induced learning and memory disorders in mice, and its mechanism may be related to enhanced cholinergic system function and reduced free radical-induced damage in brain tissues of mice.

Key words: water soluble flavonoids from Coreopsis tinctoria flowers, learning and memory abilities, acetylcholine, superoxide dismutase, malondialdehyde

中图分类号:

TS201.4

田勇，周督，邹双忆，孙星宇，郅琦，李福香，明建. 昆仑雪菊水溶性黄酮提取物对D-半乳糖致衰老小鼠学习记忆障碍的改善作用[J]. 食品科学, 2019, 40(1): 162-168.

TIAN Yong, ZHOU Du, ZOU Shuangyi, SUN Xingyu, ZHI Qi, LI Fuxiang, MING Jian. Water Soluble Flavonoids from Coreopsis tinctoria Flowers Improve Learning and Memory Ability in D-Galactose-Induced Aging Mice[J]. FOOD SCIENCE, 2019, 40(1): 162-168.

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昆仑雪菊水溶性黄酮提取物对D-半乳糖致衰老小鼠学习记忆障碍的改善作用

Water Soluble Flavonoids from Coreopsis tinctoria Flowers Improve Learning and Memory Ability in D-Galactose-Induced Aging Mice

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