食品科学

• 工艺技术 • 上一篇    下一篇

正交试验优化黑玉米籽粒中花色苷的提取

王 旭,覃鸿妮,袁文娟,王久光,王国强,刘志斋,蔡一林   

  1. 1.西南大学玉米研究所,南方山地农业教育部工程研究中心,重庆 400715;
    2.苏州工业园区服务外包职业学院,江苏 苏州 215123
  • 出版日期:2013-06-25 发布日期:2013-06-17

Optimization of Extraction Conditions for Anthocyanin Anthocyanins from Dark Maize

WANG Xu,QIN Hong-ni,YUAN Wen-juan,WANG Jiu-guang,WANG Guo-qiang,LIU Zhi-zhai,CAI Yi-lin   

  1. 1. Engineering Research Center of South Upland Agriculture, Ministry of Education, Maize Research Institute, Southwest University, Chongqing 400715, China;2. Suzhou Industrial Park Institute of Services Outsourcing, Suzhou 215123, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

以西南大学新创的全黑玉米自交系SDM(super dark maize)为材料,以提高花色苷得率和纯度为目的,采用正交试验研究乙醇体积分数、固液比、提取液pH值、提取时间、提取温度5个提取条件对籽粒中花色苷得率和纯度的影响。结果表明:在90%的乙醇、固液比1:15(g/mL)、pH1、80℃保温15h的条件下,花色苷得率最高,但纯度最低,此条件下的纯度衡量指标A550nm/A275nm为0.10,得率为18.71%;在保证花色苷纯度较高的前提下,考虑最高得率,最优提取条件为:90%的乙醇、固液比1:15、pH1.5、70℃保温15h。

关键词: 黑玉米, 花色苷得率, 花色苷纯度, 提取条件, 优化

Abstract:

An orthogonal array design was applied to establish the optimum conditions of ethanol concentration, solidto-
liquid ratio, solvent pH, temperature and time for increased yield and purity of anthocyanins from grains of Super Dark
Maize (SDM). The results showed that maximum yield (18.71%) but minimum purity (A550nm/A275nm = 0.10) of anthocyanins
were observed under the conditions of 90%, 1:15 (g/mL), 1, 80 ℃ and 15 h for ethanol concentration, solid-to-liquid ratio, pH,
temperature and and time, respectively. The optimum extraction conditions to achieve maximum yield while increasing the purity of
anthocyanins were 90% ethanol acidified to pH 1.5 as the extraction solvent with a solid-to-liquid ratio of 1:15, 70 ℃ and 15 h.

Key words: dark maize, anthocyanin yield, anthocyanin purity, extraction condition, optimization