食品科学 ›› 2013, Vol. 34 ›› Issue (13): 71-74.doi: 10.7506/spkx1002-6630-201313015

• 基础研究 • 上一篇    下一篇

孔雀石绿在南美白对虾苗中的消除规律

杨金兰,陈培基,李刘冬*,黎智广,杨宏亮   

  1. 中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,
    农业部水产品贮存保鲜质量安全风险评估实验室(广州),广东 广州 510300
  • 收稿日期:2012-03-19 修回日期:2013-05-08 出版日期:2013-07-15 发布日期:2013-06-28
  • 通讯作者: 李刘冬 E-mail:168LLd@163.com
  • 基金资助:

    国家公益性行业(农业)科研专项(nyhyzx07-047)

Accumulation and Elimination of Malachite Green in Penaeus vannamei Boone Fingerlings

YANG Jin-lan,CHEN Pei-ji,LI Liu-dong*,LI Zhi-guang,YANG Hong-liang   

  1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for
    Aquatic Product on Storage and Preservation (Guangzhou), Ministry of Agriculture, South China Sea Fisheries Research Institute,
    Chinese Academy of Fishery Sciences, Guangzhou 510300, China
  • Received:2012-03-19 Revised:2013-05-08 Online:2013-07-15 Published:2013-06-28
  • Contact: LI Liu-dong E-mail:168LLd@163.com

摘要:

为了解孔雀石绿在虾苗中的蓄积与消除规律,达到对孔雀石绿的禁用监控。本实验对初始体长约0.9cm的 南美白虾苗以0.15μg/mL质量浓度孔雀石绿进行药浴2h,再转移到室外自然环境中进行养殖,并连续采样监测。将 取得的样品采用高效液相色谱-串联质谱检测其孔雀石绿及其代谢产物隐色孔雀石绿的残留状况。结果表明:孔雀 石绿在南美白虾苗中代谢较快,其含量降至检测限以下只需要3d。而其代谢物隐色孔雀石绿存留时间较长,消除过 程缓慢,需长达9d,可以做为检测的标志物。

关键词: 孔雀石绿, 隐色孔雀石绿, 南美白对虾苗, 消除规律

Abstract:

The accumulation and elimination of malachite green (MG ) was studied in Penaeus vannamei boone fingerlings
with the purpose to monitor its use in aquaculture. Penaeus vannamei boone fingerlings 0.9 cm long were exposed to MG
at a concentration of 0.15 μg/mL for 2 h under natural environment. Dynamic changes of MG in Penaeus vannamei boone
fingerlings were monitored by HPLC-MS. The result showed that MG was eliminated rapidly in body and reached below
LOD after 3 d, but leucomalachite green (LMG) was eliminated very slowly and reached below LOD after 9 d. So it can be
used as a target analyte in residue-monitoring programs.

Key words: malachitegreen, leucomalachite green, Penaeus vannamei boone fingerlings, elimination

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