膳食核苷酸长期喂养对SD大鼠肝增龄性纤维增生的作用和机制

doi:10.7506/spkx1002-6630-201315064

食品科学

膳食核苷酸长期喂养对SD大鼠肝增龄性纤维增生的作用和机制

徐美虹，李勇*

北京大学公共卫生学院营养与食品卫生学系，北京 100191

出版日期:2013-08-15 发布日期:2013-09-03
通讯作者: 李勇

Anti-fibrosis Effects of Long-term Oral Administration of Dietary Nucleotides in the Chronologically Aging Liver of Sprague-dawley Male Rats

XU Mei-hong，LI Yong*

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China

Online:2013-08-15 Published:2013-09-03
Contact: LI Yong

摘要/Abstract

摘要：

目的：探讨长期喂养膳食核苷酸(NTs)对SD大鼠由增龄引起的肝纤维增生的保护作用和机制。方法：初断乳雄性大鼠随机分为老年对照组、0.01%和0.64% NTs干预组，每组6只，喂养24～26月后进行实验；另取8只12月龄对照组SD大鼠作为中年对照组。采用miRNA基因芯片对各组肝脏的miRNA表达变化，同时用qRT-PCR检测了相关靶基因的表达水平，并对肝脏中羟脯氨酸水平进行测定。结果：NTs可显著调控肝组织内11个miRNA的表达。其中miR-182基因显著上调，其降低靶基因Thbs-2的表达，降低细胞外基质纤维化；miR-328a*基因显著下调，降低Pdgf-b基因的表达，降低与肝纤维化形成有关的重要细胞因子的分泌，减轻肝星状细胞的增殖。与此同时，NTs长期干预可一定程度降低增龄引起的肝脏中羟脯氨酸水平上升。结论： NTs长期干预可缓解由增龄引起的肝纤维增生。

关键词: 膳食核苷酸 (NTs), 衰老肝脏, miRNA

Abstract:

Purpose: To investigate the anti-fibrosis effects of dietary nucleotides on aging liver and explore their
possible mechanisms. Methods: Liver samples from rats at the age of 24－26 months (aged control, 0.01% NTs and
0.64% NTs; n = 6/group) were excised for analysis. Another group of male SD rats (n = 8) which were fed the control diet
were sacrificed at the age of 12 months to be used as middle aged controls. The liver samples were used for investigation on
Hyp level, as well as the expression levels of miRNA and their target genes. Results: This study established the association
of miR-182 and miR-328a* with NTs in aging liver, meanwhile highlighted the correlation of miRNA expression with their
target genes Thbs-2 and Pdgf-b. This study also identified that long term administration of NTs inhibited the age related
increase of Hyp level in the chronologically aged liver. Conclusions: The present study demonstrated that the anti-fibrosis
effects of long-term NTs on chronological liver aging. It is possible because NTs affect miR-182 and miR-328a* through
their target genes Thbs-2 and Pdgf-b in the aged liver of SD male rats.

Key words: dietary nucleotides (NTs), aging liver, miRNA

中图分类号:

R151.3

徐美虹，李勇*. 膳食核苷酸长期喂养对SD大鼠肝增龄性纤维增生的作用和机制[J]. 食品科学, doi: 10.7506/spkx1002-6630-201315064.

XU Mei-hong，LI Yong*. Anti-fibrosis Effects of Long-term Oral Administration of Dietary Nucleotides in the Chronologically Aging Liver of Sprague-dawley Male Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201315064.

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膳食核苷酸长期喂养对SD大鼠肝增龄性纤维增生的作用和机制

Anti-fibrosis Effects of Long-term Oral Administration of Dietary Nucleotides in the Chronologically Aging Liver of Sprague-dawley Male Rats

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