食品科学 ›› 2013, Vol. 34 ›› Issue (21): 172-176.doi: 10.7506/spkx1002-6630-201321036

• 生物工程 • 上一篇    下一篇

产果糖基转移酶的黑曲霉细胞固定化条件优化

张 颖1,姚盟成2,荆 玮2,路福平2,李 玉2,*   

  1. 1.中国环境管理干部学院生态学系,河北 秦皇岛 066004;2.天津科技大学生物工程学院,天津 300457
  • 收稿日期:2012-09-06 修回日期:2013-09-26 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 李玉 E-mail:liyu@tust.edu.cn
  • 基金资助:

    “十二五”农村领域国家科技计划项目(2011AA100905-4);天津市科技计划项目(10ZCKFNC01700);
    教育部“长江学者和创新团队发展计划”项目(IRT1166);国家“863”计划项目(2012AA021502)

Optimization of Immobilization of Aspergillus niger Cells with Fructosyltransferase Activity

ZHANG Ying1,YAO Meng-cheng2,JING Wei2,LU Fu-ping2,LI Yu2,*   

  1. 1. Department of Ecology, Environmental Management College of China, Qinhuangdao 066004, China;
    2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
  • Received:2012-09-06 Revised:2013-09-26 Online:2013-11-15 Published:2013-10-28
  • Contact: LI Yu E-mail:liyu@tust.edu.cn

摘要:

以卡拉胶为固定化载体,对含有果糖基转移酶的黑曲霉细胞进行固定化。通过单因素和Box-Behnken试验,得到其最优固定化工艺为:卡拉胶9.4g/L、多乙烯多胺9g/L、戊二醛10g/L、KCl 15g/L、菌浓100g/L,在此最优工艺条件下低聚果糖含量达到最大值40.12%,与模型预测值接近。优化后的工艺可为低聚果糖的生产提供技术参考。

关键词: 低聚果糖, 黑曲霉, 果糖基转移酶, 固定化细胞, 响应面试验

Abstract:

Aspergillus niger cells with fructosyltransferase were immobilized by entrapping into carrageenan. Five immobilization
parameters were primarily studied by one-factor-at-a-time method, and the optimization of three of them (concentration of carrageenan,
glutaraldehyde and polyethylene polyamine, PEPA) was explored by using a Box-Behnken design coupled with response surface
methodology. The results showed the optimal process conditions were 9.4 g/L carrageenan, 10 g/L glutaraldehyde, 15 g/L KCl, 9 g/L PEPA and
100 g/L Aspergillus niger cells. Under the optimal conditions, the maximum yield of fructooligosaccharides (FOS)
was 40.12%, which was consistent with the predicted value, and the established model was reliable in prediction.

Key words: fructooligosaccharide, Aspergillus niger, fructosyltransferase, immobilized cells, response surface methodology

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