苦瓜改善胰岛素抵抗功能与作用机制研究进展

doi:10.7506/spkx1002-6630-201321073

摘要/Abstract

摘要：

苦瓜富含多种生物活性成分，其调脂降糖作用一直以来都是国内外学者研究的热点。本文以苦瓜改善脂肪组织、骨骼肌和肝脏的胰岛素抵抗为分析对象，综述近年来苦瓜改善胰岛素抵抗的功能及其可能作用机制的研究进展，并进一步对其作用机制的深入研究进行展望，最终为苦瓜健康食品的开发提供参考。

关键词: 苦瓜, 胰岛素抵抗, 靶组织, 调脂降糖, 研究进展

Abstract:

Momordica charantia L. contains many biologically active components. Domestic and international
researchers have paid great attention to its blood lipid- and glucose-lowing effects. In this review, three types of insulin
target tissues (adipose tissue, skeletal muscle and liver) are analyzed, and the functions of Momordica charantia L. for
ameliorating insulin resistance of the target tissues and its possible mechanisms are also reviewed. In addition, further
studies on its mechanism are proposed. Therefore, this review can provide the basis for the development of healthy
foods containing Momordica charantia L..

Key words: Momordica charantia L., insulin resistance, target tissue, blood lipid- and glucose-lowing effect, research progress

中图分类号:

R151.3

董英，钱希文，白娟，张易，祝莹. 苦瓜改善胰岛素抵抗功能与作用机制研究进展[J]. 食品科学, 2013, 34(21): 369-374.

DONG Ying，QIAN Xi-wen，BAI Juan，ZHANG Yi，ZHU Ying. Research Progress in Functions and Mechanisms of Momordica charantia L. for Ameliorating Insulin Resistance[J]. FOOD SCIENCE, 2013, 34(21): 369-374.

参考文献

[1] Smyth S. Heron A. Diabetes and obesity: the twin epidemics[J]. Nature Medicine, 2006, 12: 75-80.
[2] Moller D E. New drug targets for type 2 diabetes and the metabolic syndrome[J]. Nature, 2001, 414: 821-827.
[3] Kumar S R, Ashish J, Satish N. Momordica charantia Linn.: A mini review[J]. International Journal of Biomedical Research, 2011, 11(2): 579-587.
[4] 徐斌, 董英, 张慧慧等. 苦瓜多糖对链脲佐菌素诱导糖尿病小鼠的降血糖效果[J]. 营养学报, 2006, 28(4): 401-403.
[5] 董英, 张慧慧. 苦瓜多糖降血糖活性成分的研究[J]. 营养学报, 2008, 30(1): 54-56.
[6] 董英, 朱晶英, 陈钧. 苦瓜伤流液对链脲佐菌素诱导糖尿病小鼠的降糖功能研究[J]. 食品科学, 2010, 31(21): 349-352.
[7] 刘峻瑒, 贺伊博, 叶菲. 半合成高脂饲料和合成高脂饲料对大鼠糖脂代谢的影响[J]. 中国药学杂志, 2011, 46(10): 753-756.
[8] Shih C C, Lin C H, Lin W L. Effects of Momordica charantia on insulin resistance and visceral obesity in mice on high-fat diet[J]. Diabetes Research and Clinical Practice, 2008, 81: 134-143.
[9] Ying Zhu, Ying Dong, Xiwen Qian, et al. Effect of superfine grinding on antidiabetic activity of bitter melon powder[J]. International Journal of Molecular Sciences,2012,13, 14203-14218.
[10] Popovich D G, Lee Y, Li Lu, et al. Momordica charantia seed extract reduces pre-adipocyte viability, affects lactate dehydrogenase release, and lipid accumulation in 3T3-L1 cells[J]. Journal of Medicinal Food, 2011, 14(3): 201-208.
[11] 张怡君. 苦瓜籽油延缓体脂肪生成机制研究[D/OL]. http://ir.cmu.edu.tw/ir/handle/ 310903500/41336, 2011-10-17/2012-9-5.
[12] Ng, T B, Wong C M, Li W W, et al. Acid-ethanol extractable compounds from fruits and seeds of the bitter gourd Momordica charantia: effects on lipid metabolism in isolated rat adipocytes. American Journal of Chinese Medicine, 1987, 15: 31–42.
[13] Meir P, Yaniv Z. An in vitro study on the effect of Momordica charantia on glucose uptake and glucose metabolism in rats. Planta Medica,1984, 51: 12–16.
[14] Roffey B W C, Atwal A S, Johns T, et al. Water extracts from Momordica charantia increase glucose uptake and adiponectin secretion in 3T3-L1 adipose cells[J]. Journal of Ethnopharmacology, 2007, 112: 77-84.
[15] Karunanayake E, Jeevathayaparan S, Tennekoon K. Effect of Momordica charantia fruit juice on streptozotocin-induced diabetes in rats[J]. Journal of Ethnopharmacology, 1990, 30: 199–204.
[16] Ali L, Azad Khan A, Mamun M, et al. Studies on hypoglycemic effects of fruit pulp, seed, andwhole plant of Momordica charantia on normal and diabeticmodel rats[J]. Planta Medica, 1993, 59: 408–412.
[17] Amer P. The adipocyte in insulin resistance: key molecules and the impact of the thiazolidinediones[J]. Trends Endocrinol. Metab, 2003, 14(3): 137-145.
[18] 秦培洁, 仝小林. 肥胖的瘦素抵抗机制研究进展[J]. 医学综述, 2010, 16(5): 662-665.
[19] Chen Q X, Chan L L Y, Li E T S. Bitter melon (Momordica charantia) reduces adiposity, lowers serum insulin and normalizes glucose tolerance in rats fed a high fat diet[J]. The Journal of Nutrition, 2003, 133: 1088-1093.
[20] Maeda N, Shimomura I, Kishida K, et al. Diet-induced insulin resistance in mice lacking adiponectin/ ACRP30[J]. Natural Medicine, 2002, 8: 731-737.
[21] Shiha C C, Lin C H, Lin W L, et al. Momordica charantia extract on insulin resistance and the skeletal muscle GLUT4 protein in fructose-fed rats[J]. Journal of Ethnopharmacology, 2009, 123: 82-90.
[22] Popovich D G, Li L, Zhang W. Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells[J]. Food and Chemical Toxicology, 2010, 48: 1619-1626.
[23] Rangwala, Lazer S M, et al. Perxisome proliferator-activated receptor gamma in diabetes and metabolism[J]. Trends in Pharmacological Sciences, 2004, 25: 331-336.
[24] Huang H L, Hong Y W, Wong Y H, et al. Bitter melon (Momordica charantia L.) inhibits adipocyte hypertrophy and down regulates lipogenic gene expression in adipose tissue of diet-induced obese rats[J]. British Journal of Nutrition, 2008, 99: 230-239.
[25] Nerurkar P V, Lee Y K, Nerurkar V R. Momordica charantia (bitter melon) inhibits primary human adipocyte differentiation by modulating adipogenic genes[J]. BMC Complementary and Alternative Medicine, 2010, 10(1): 34-43.
[26] Munzer T, Harman S M, Hees P, et al. Effect of GH and/or sex steroid administration on obdominal subcutaneous and visceral fat in health aged women and men[J]. Journal of Clinical Endocrinology and Metabolism, 2001, 86: 3604-3610.
[27] Perseghin G, Scifo P, De C F, et al. Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in human: a 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents[J]. Diabetes, 1999, 48:1600-1606.
[28] Chen Q X, Li E T S. Reduced adiposity in bitter melon (Momordica charantia) fed rats is associated with lower tissue triglyceride and higher plasma catecholamines[J]. British Journal of Nutrition, 2005, 93: 747-754.
[29] Wang Z Q, Zhang X H, Yu Y M, et al. Bioactives from bitter melon enhance insulin signaling and modulate acyl carnitine content in skeletal muscle in high-fat diet-fed mice[J]. Journal of Nutritional Biochemistry, 2011, 22(11): 1064-1073.
[30] Singh J, Hundal H S, Wackerhage H, et al. Momordica charantia fruit juice stimulates glucose and amino acid uptakes in L6 myotubes[J]. Molecular and Cellular Biochemistry, 2004, 261: 99-104.
[31] Kelley D E, He J, Menshikova E V, et al. Dysfunction in mitochondria in human skeletal muscle in type 2 diabetes[J]. Diabetes, 2002, 51: 2944–2950.
[32] Chan L L Y, Chen Q X, Go A G G, et al. Reduced adiposity in bitter melon (Momordica charantia)–fed rats is associated with increased lipid oxidative enzyme activities and uncoupling protein expression[J]. Biochemical and Molecular Actions of Nutrients, 2005, 135(11): 2517-2523.
[33] Kellerer M, Lammers R, Hu H?ring. Insulin signal transduction: possible mechanism for insulin resistance[J]. Experimental and Clinical Endocrinology and Diabetes, 1999, 107(2): 97-106.
[34] 程媛, 王佑民, 丁晓洁. 肥胖大鼠骨骼肌 AMPK表达及其与糖脂代谢的关系[J]. 安徽医科大学学报, 2010, 45(2): 180-182.
[35] Tan M J, Ye J M, Turner N, et al. Antidiabetic activities of triterpenoids isolated from bitter melon associated with activation of the AMPK pathway[J]. Chemistry and Biology, 2008, 15: 263-273.
[36] Kumar R, Balaji S, Uma T S, et al. Fruit extracts of Momordica charantia potentiate glucose uptake and up-regulate Glut-4,PPARγ and PI3K[J]. Journal of Ethnopharmacology, 2009, 126(3): 533-537.
[37] Sridhar M G, Vinayagamoorthi R, Suyambunathan V A, et al. Bitter gourd (Momordica charantia) improves insulin sensitivity by increasing skeletal muscle insulin-stimulated IRS-1 tyrosine phosphorylation in high-fat-fed rats[J]. British Journal of Nutrition, 2008, 99: 806-812.
[38] Jayasooriya A P, Sakono M, Yukizaki C, et al. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets[J]. Journal of Ethnopharmacology, 2000, 72: 331-336.
[39] Senanayake G V K, Maruyama M, Shibuya K, et al. The effects of bitter melon (Momordica charantia) on serum and liver triglyceride levels in rats[J]. Journal of Ethnopharmacology, 2004, 91: 257-262.
[40] Szczepaniak L S, Dobbins R L, Stein D T, et al. Bulk magnetic susceptibility effects on assessment of intra- and extramycellular lipids in vivo[J]. Magnetic Reasonance in Medicine, 2002, 47(3): 607—610.
[41] 盛清凯, 赵红波, 刘俊珍等. 苦瓜降糖多肽-p对2型糖尿病模型大鼠血糖血脂的影响[J]. 山东农业大学学报(自然科学版), 2008, 39(1): 23-25.
[42] 钱希文, 祝莹, 张易等. 苦瓜冻干超微粉改善2型糖尿病大鼠糖脂代谢紊乱的研究[A]. 华东六省一市生物化学与分子生物学会学术交流会论文集[C], 江苏徐州, 2012.7.
[43] Senanayake G V K, Nobuhiro F, Shoko N, et al. Mechanisms underlying decreased hepatic triacylglycerol and cholesterol by dietary bitter melon extract in the rat[J]. Lipids, 2012, 47: 495–503.
[44] Nerurkar P V, Lee Y K, Motosue M, et al. Momordica charantia (bitter melon) reduces plasma apolipoprotein B-100 and increases hepatic insulin receptor substrate and phosphoinositide-3 kinase interactions[J]. British Journal of Nutrition, 2008, 100: 751-759.
[45] Chao Cheyi, Huang Chingjang. Bitter gourd (Momordica charantia) extract activates peroxisome proliferator-activated receptors and up regulates the expression of the acyl CoA oxidase gene in H4IIEC3 hepatoma cells[J]. Journal of Biomedical Science, 2003, 10: 782-791.
[46] Chang Chii, Tseng Hsini, Liao Yunwen, et al. In vivo and in vitro studies to identify the hypoglycaemic constituents of Momordica charantia wild variant WB24[J]. Food Chemistry, 2011, 125: 521-528.
[47] Alcorn T, Ouyang Y D. Diabetes saps health and wealth from China’s rise[J]. The Lancet, 2012, 379(9833): 2227-2228.
[48] Oresic M. Metabolomics, a novel tool for studies of nutrition, metabolism and lipid dysfunction[J]. Nutrition, Metabolism and Cardiovascular Diseases, 2009, 19: 816-824.
[49] Rezzi S, Ramadan Z, Fay L B, et al. Nutritional metabonomics: applications and perspectives[J]. Journal of Proteome Research, 2007, 6: 513-525.