食品科学

• 基础研究 • 上一篇    下一篇

条斑紫菜多糖的纯化、理化性质及其免疫活性研究

邱伟芬1,杨文建1,薛 梅1,杨方美2,赵清秋2,方 勇1,马 宁1,安辛欣2,赵立艳2,胡秋辉2,*   

  1. 1.南京财经大学食品科学与工程学院,江苏 南京 210023; 2.南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2013-12-15 发布日期:2014-01-03
  • 通讯作者: 胡秋辉
  • 基金资助:

    国家“863”计划项目(2007AA100403)

Purification, Physicochemical Properties and Immunomodulatory Activity of Polysaccharides from Porphyra yezoensis

QIU Wei-fen1,YANG Wen-jian1,XUE Mei1,YANG Fang-mei2,ZHAO Qing-qiu2,FANG Yong1,MA Ning1, AN Xin-xin2,ZHAO Li-yan2,HU Qiu-hui2,*   

  1. 1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China;
    2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2013-12-15 Published:2014-01-03
  • Contact: HU Qiu-hui

摘要:

利用DEAE 纤维素-52和Sephadex G-100凝胶柱层析对木瓜蛋白酶法提取的条斑紫菜多糖进行分离纯化,得到PYP-1、PYP-2、PYP-3和PYP-4多糖组分。然后对PYP-2、PYP-3和PYP-4单一组分的总糖含量、糖醛酸含量、硫酸基含量、分子质量、单糖组成和红外光谱特征进行分析,并运用小鼠单核巨噬细胞RAW264.7模型研究各组分的体外免疫调节活性。结果表明:PYP-2、PYP-3和PYP-4组分总糖含量分别为91.83%、96.93%和97.66%,糖醛酸含量分别为19.39%、23.77%和18.23%,硫酸基含量分别为18.84%、17.63%和18.41%,分子质量分别为478.6、314.4、417.3kD,且3种多糖均是由半乳糖、葡萄糖和木糖3种单糖以β-糖苷键链接的吡喃糖。3种多糖在25~250μg/mL质量浓度范围内能够显著促进小鼠腹腔巨噬细胞RAW264.7的增殖,增强其吞噬中性红能力,表现出显著的免疫调节活性。

关键词: 条斑紫菜, 多糖, 纯化, 理化性质, 免疫活性

Abstract:

Polysaccharides from Prophyra yezoensis (PYP) were extracted with papain and purified using DEAE
cellulose-52 and Sephadex G-100 chromatography. Four fractions named as PYP-1, PYP-2, PYP-3 and PYP-4 were
obtained, and their physicochemical properties including total sugar content, molecular weight, sulfate and uronic acid
contents and monosaccharide composition were investigated. In addition, RAW264.7 macrophage cell model was employed
to evaluate the immunomodulatory activity in vitro. Results showed that total sugar contents of PYP-2, PYP-3 and PYP-4
were 91.83%, 96.93% and 97.66%, respectively. Uronic acid contents were 19.39%, 23.77% and 18.23%, respectively, and
sulfate group contents were 18.84%, 17.63% and 18.41%, respectively. All these three polysaccharides were composed of
galactose, glucose and xylose with molecular weight of 478.6, 314.4 kD, and 417.3 kD, respectively. Pyranose ring structure
and β-type glycosidic linkages were also found in them. PYP-2, PYP-3, and PYP-4 when used at a concentration ranging
from 25 to 250 μg/mL could significantly promote the proliferation and neutral red phagocytosis of RAW264.7 macrophage
cells in vitro, suggesting the potential immunomodulatory activities of PYP-2, PYP-3, and PYP-4.

Key words: Porphyra yezoensis, polysaccharides, purification, physicochemical properties, immunomodulatory activity

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