黑灵芝葡聚糖高活性硫酸化产物的制备及分离纯化

doi:10.7506/spkx1002-6630-201323027

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 128-132.doi: 10.7506/spkx1002-6630-201323027

黑灵芝葡聚糖高活性硫酸化产物的制备及分离纯化

张汇，王君巧，聂少平，张莘莘，王远兴，谢明勇*

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-07-06 修回日期:2013-10-30 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 谢明勇 E-mail:xmync@sina.com
基金资助:
江西省研究生创新专项资金项目(YC2012-B003)；国家自然科学基金地区项目(21265011)；
国家自然科学基金重点项目(31130041)；“十二五”国家科技支撑计划项目(2012BAD33B06)

Preparation and Isolation of Bioactive Sulfated Derivatives from Water-insoluble Glucan in Ganoderma arum

ZHANG Hui，WANG Jun-qiao，NIE Shao-ping，ZHANG Shen-shen，WANG Yuan-xing，XIE Ming-yong*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2013-07-06 Revised:2013-10-30 Online:2013-12-15 Published:2014-01-03
Contact: XIE Ming-yong E-mail:xmync@sina.com

摘要/Abstract

摘要：

目的：通过化学修饰手段改善黑灵芝葡聚糖的水溶解性，探讨修饰产物的抗肿瘤活性及溶液性质。方法：采用氯磺酸-吡啶法制备不同酯化度的硫酸化修饰产物(AGAP)，通过凝胶渗透色谱和红外光谱进行表征，MTT法检测对S-180肉瘤细胞毒性，筛选具有高活性的硫酸化多糖；对高活性硫酸化多糖进行分离纯化，并研究其溶液性质。结果：制备了9种不同酯化度的修饰产物，筛选出酯化度(DS)为1.14的硫酸化多糖GAPS5，其糖含量为66.3%，对S-180肉瘤细胞生长的抑制率达到67.74%。凝胶柱分离得到高纯度的AGAPS5-1组分，其在0.1mol/LNaNO3溶液中的重均分子质量(Mw)和数均分子质量(Mn)分别为5.19×104u和2.63×104u，呈多分散性，以无规线团状形式存在。结论：硫酸化修饰能改善黑灵芝葡聚糖的水溶解性及溶液性质，增强抗肿瘤活性。

关键词: 黑灵芝, 葡聚糖, 硫酸化, 抗肿瘤, 分离纯化

Abstract:

Objective: To improve the water solubility of a water-insoluble glucan from Ganoderma atrum by sulfated
modification technique and to investigate the in vitro antitumor activity and properties of sulfated derivatives. Methods: The
water-insoluble glucan was modified using chlorosulfonic acid-pyridine (CSA-Pyr) method to obtain sulfated products with
different degrees of sulfation (DS). The sulfated polysaccharides were characterized by gel filtration chromatography and
FT-IR spectroscopy. The cytotoxicity on S-180 sarcoma cells was measured to screen a fraction with significant antitumor
activity. The separated fraction with high antitumor activity was purified and its solution was subjected to molecular
characterization. Results: Nine sulfated products with different DS were prepared. AGAPS5 (DS 1.14) had significant
antitumor activity. The sugar content of AGAPS5 was 66.3%, and the inhibitory rate of AGAPS5 on the growth of S-180
sarcoma cells was 67.74%. The purified AGAPS5-1 showed a random coil chain in 0.1 mol/L NaNO3 solution. The weight-average
molecular weight (Mw) of AGAPS5-1 was 5.19 × 104 u and the number-average molecular weight (Mn) was 2.63 × 104 u. Conclusion:
Sulfated modification can improve the water-solubility and solution properties, and enhance the in vitro antitumor activity of
glucan from Ganoderma atrum.

Key words: Ganoderma atrum, glucan, sulfated modification, antitumor, isolation

中图分类号:

Q949.32

张汇，王君巧，聂少平，张莘莘，王远兴，谢明勇*. 黑灵芝葡聚糖高活性硫酸化产物的制备及分离纯化[J]. 食品科学, 2013, 34(23): 128-132.

ZHANG Hui，WANG Jun-qiao，NIE Shao-ping，ZHANG Shen-shen，WANG Yuan-xing，XIE Ming-yong*. Preparation and Isolation of Bioactive Sulfated Derivatives from Water-insoluble Glucan in Ganoderma arum[J]. FOOD SCIENCE, 2013, 34(23): 128-132.

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黑灵芝葡聚糖高活性硫酸化产物的制备及分离纯化

Preparation and Isolation of Bioactive Sulfated Derivatives from Water-insoluble Glucan in Ganoderma arum

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