酒类酒球菌中β-葡萄糖苷酶性质研究

doi:10.7506/spkx1002-6630-201323043

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 206-211.doi: 10.7506/spkx1002-6630-201323043

酒类酒球菌中β-葡萄糖苷酶性质研究

杨芮1，吕珍1，文彦1，李凯1，朱成龙1，刘树文1,2,*

1.西北农林科技大学葡萄酒学院，陕西杨凌 712100；2.陕西省葡萄与葡萄酒工程技术研究中心，陕西杨凌 712100

收稿日期:2012-12-02 修回日期:2013-11-06 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 刘树文 E-mail:liushuwen@nwsuaf.edu.cn
基金资助:
“十二五”国家科技支撑计划项目(2012BAD31B07)；中央高校基本科研业务费专项(2109021104)；
国家现代农业产业技术体系建设专项(nycytx-30-ch-03)

β-Glycosidase Activity of Oenococcus oeni

YANG Rui1，LÜ Zhen1，WEN Yan1，LI Kai1，ZHU Cheng-long1，LIU Shu-wen1,2,*

1. College of Enology, Northwest A&F University, Yangling 712100, China；
2. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China

Received:2012-12-02 Revised:2013-11-06 Online:2013-12-15 Published:2014-01-03
Contact: LIU Shu-wen E-mail:liushuwen@nwsuaf.edu.cn

摘要/Abstract

摘要：

以26株野生酒类酒球菌为研究对象，通过测定β-葡萄糖苷酶活性筛选出8株高酶活力菌株。进而以2株商业菌株为对照，分析温度、pH值、酒精体积分数、葡萄糖、果糖以及模拟酒条件对8株高酶活菌株中β-葡萄糖苷酶活性的影响。结果表明：CS-1b酶活性质最优，其酶活性质为：最适pH值为5，最适温度为30℃，在葡萄酒pH值和温度下均有较高酶活；高酒精度对该酶有强烈抑制作用；低质量浓度葡萄糖和果糖对该酶有轻微激活作用，高质量浓度葡萄糖和果糖均有轻微抑制作用；菌株CS-1b在模拟酒条件下可保持较高酶活力3.755μmol/(g·min)，相对酶活力为34.55%。

关键词: 葡萄酒；酒类酒球菌；&beta, -葡萄糖苷酶；酶活性

Abstract:

In this study, 8 strains of Oenococcus oeni with the highest β-glycosidase activity were obtained by measuring
β-glycosidase activity in 26 wild Oenococcus oeni strains and the effects of temperature, pH, alcohol concentration,
glucose, fructose and wine-model conditions on β-glycosidase activity from the selected strains were investigated using
two commercial strains as controls. The results showed that strain CS-1b exhibited the best enzymatic properties among the
tested strains under the optimum conditions of pH 5 and 30 ℃ and it retained high β-glycosidase activity under wine-model
conditions of pH and temperature. The β-glycosidase activity was slightly activated by lower concentrations of glucose or
fructose but slightly inactivated at higher concentrations. Higher concentrations of alcohol strongly inhibited its activity.
Under wine-model conditions, the β-glycosidase activity of this strain was 3.755 μmol/(g·min), accounting for 34.55% of
the original activity.

Key words: wine；Oenococcus oeni；&beta, -glycosidase；enzyme activity

中图分类号:

Q815

杨芮1，吕珍1，文彦1，李凯1，朱成龙1，刘树文1,2,*. 酒类酒球菌中β-葡萄糖苷酶性质研究[J]. 食品科学, 2013, 34(23): 206-211.

YANG Rui1，Lü Zhen1，WEN Yan1，LI Kai1，ZHU Cheng-long1，LIU Shu-wen1,2,*. β-Glycosidase Activity of Oenococcus oeni[J]. FOOD SCIENCE, 2013, 34(23): 206-211.

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酒类酒球菌中β-葡萄糖苷酶性质研究

β-Glycosidase Activity of Oenococcus oeni

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