关键词: 赭曲霉毒素A, 葡萄酒, 高效液相色谱法, 酶联免疫吸附测定法, 方法优化

Abstract:

The purpose of this study was to establish the optimal sample pretreatment procedure and analytical method for
determining ochratoxin A (OTA) in wine. Sample pretreatment was achieved by alternative methods, either by liquid-liquid
extraction or by immunoaffinity column chromatography (IAC) and two different analytical methods, high-performance
liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), were compared for their suitability
for large-scale determination of OTA in wine from the viewpoints of OTA recovery, cost, efficiency and environmental
protection. The selected analytical procedure was validated by applying it for the determination of wines imported from
France, Spain, Italy and Australia as well as domestic wines. Based on the results of this study, liquid-liquid extraction in
combination with ELISA is a more suitable method for large-scale screening of wines for OTA and can be validated by
comparative determination of the positive samples using HPLC analysis following IAC clean-up. The method proposed in
this study is rapid, convenient, sensitive and accurate and meets the analytical requirements of recovery and precision.

Key words: ochratoxin A, wine, high performance liquid chromatography (HPLC), enzyme-linked immuno sorbent assay (ELISA), optimization