食品科学 ›› 2014, Vol. 35 ›› Issue (1): 199-203.doi: 10.7506/spkx1002-6630-201401039

• 生物工程 • 上一篇    下一篇

一株产L-木酮糖菌株的分离筛选及鉴定

张玉宝1,2,赵祥颖3,杨丽萍3,韩延雷3,刘建军1,3,*   

  1. 1.山东农业大学食品科学与工程学院,山东 泰安 271018;2.山东省兽药添加剂工程技术研究中心,山东 济南
    250306;3.山东省食品发酵工程重点实验室,山东 济南 250013
  • 收稿日期:2012-12-18 修回日期:2013-11-22 出版日期:2014-01-15 发布日期:2014-01-22
  • 通讯作者: 刘建军 E-mail:zhangyubao0201@163.com

Isolation and Identification of a Novel L-Xylulose-Producing Strain

ZHANG Yu-bao1,2, ZHAO Xiang-ying3, YANG Li-ping3, HAN Yan-lei3, LIU Jian-jun1,3,*   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;
    2. Shandong Veterinary Medicine Additive Engineering Technology Research Center, Jinan 250306, China;
    3. Key Laboratory of Food and Fermentation Engineering of Shandong Province, Jinan 250013, China
  • Received:2012-12-18 Revised:2013-11-22 Online:2014-01-15 Published:2014-01-22
  • Contact: LIU Jian-jun E-mail:zhangyubao0201@163.com

摘要:

通过初筛和复筛从土壤中分离获得1株能够转化木糖醇生产一种稀有糖——L-木酮糖的芽孢杆菌ZN-14。利用该菌株的静息细胞以20 g/L木糖醇为底物,在pH 9.0、37 ℃条件下转化24 h,转化率达到26.62%。通过对菌株ZN-14形态特征观察、生理生化特性鉴定以及16S rDNA基因序列同源性的分析,将其鉴定为巨大芽孢杆菌(Bacillus megaterium)。

关键词: L-木酮糖, 巨大芽孢杆菌, 生物转化

Abstract:

A bacterium numbered as ZN-14 with the ability for efficient oxidization of xylitol to L-xylulose, a rare
ketopentose, was isolated and purified from soil samples, through primary isolation and secondary screening. We have
employed this isolated strain for the production of L-xylulose from xylitol by a resting cell reaction at 37 ℃ and pH 9.0.
After 24 h, L-xylulose was produced at a yield of 26.62% when 20 g/L xylitol was utilized as the precursor. The strain
was identified as Bacillus megaterium, based on the sequence analysis of 16S rDNA and the general morphological and
biochemical characteristics.

Key words: L-xylulose, Bacillus megaterium, biotransformation

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