食品科学 ›› 2014, Vol. 35 ›› Issue (2): 115-119.doi: 10.7506/spkx1002-6630-201402021

• 工艺技术 • 上一篇    下一篇

阴香花中原花青素的提取工艺

张 镜,叶春飞   

  1. 嘉应学院生命科学学院,广东 梅州 514015
  • 收稿日期:2013-07-16 修回日期:2013-12-30 出版日期:2014-01-25 发布日期:2014-02-19
  • 通讯作者: 张镜 E-mail:zhangcqf@jyu.edu.cn
  • 基金资助:

    广东省科技计划项目(2009B011300015)

Extraction of Proanthocyanidins from Flowers of Cinnamomum burmannii

ZHANG Jing, YE Chun-fei   

  1. College of Life Science, Jiaying University, Meizhou 514015, China
  • Received:2013-07-16 Revised:2013-12-30 Online:2014-01-25 Published:2014-02-19
  • Contact: ZHANG Jing E-mail:zhangcqf@jyu.edu.cn

摘要:

优化阴香花中原花青素的提取工艺。以经冷冻干燥、粉碎与80 目过筛的阴香花粉末为供试物料,丙酮- 乙醇混合提取,提取液以铁盐催化显色,测定反应体系的吸光度。在提取溶剂体积分数、pH值、料液比、温度 及时间单因素试验基础上进行正交试验优化提取条件。结果表明:以体积分数70%的混合液(70%丙酮-70%乙醇 (3∶2))为提取溶剂、pH 2、料液比1∶7.5(g/mL)、提取温度50 ℃、提取时间2 h提取阴香花原花青素的效果为 佳,以此参数条件下提取3 次原花青素的提取率为95.37%、得率为9.02%。丙酮是阴香花原花青素提取的有效溶 剂,采用优化提取条件可有效的提取阴香花原花青素。

关键词: 阴香花, 原花青素, 提取工艺

Abstract:

This study was undertaken to optimize the extraction of proanthocyanidins from flowers of Cinnamomum
burmannii. The starting raw material, freeze-dried flowers of Cinnamomum burmannii ground and sieved through a 80-
mesh sieve, was extracted using acetone-ethanol and the extract was submitted to color development catalyzed by iron salt
before measuring the absorbance. Five extraction conditions including extractant concentration, pH, solid-to-solvent ratio,
temperature and time were optimized by one-factor-at-a-time and orthogonal array design methods. The optimal extraction
efficiency was obtained by three repeated cycles of extraction at 50 ℃ for 2 h using an extractant consisting of 70% acetoneethanol
in water, as prepared by mixing 70% acetone and 70% ethanol with a volume ratio of 3:2, with a solid-to-solvent
ratio of 1:7.5 (g/mL). Under these conditions, the proanthocyanidin recovery was 95.37%, and the proanthocyanidin
content in dried flowers of Cinnamomum burmannii was 9.02%. Thus acetone is an effective solvent for extracting
proanthocyanidins from flowers of Cinnamomum burmannii under optimized conditions.

Key words: flower of Cinnamomum burmannii, proanthocyanidins, extraction process

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