食品科学

• 分析检测 • 上一篇    下一篇

高效毛细管电泳检测植物蛋白食品中的特征蛋白质

李克宏1,白彦坤1,周 巍1,张 岩1,*,庞 军2,高文惠2   

  1. 1.河北省食品质量监督检验研究院,河北 石家庄 050091;2.河北科技大学生物科学与工程学院,河北 石家庄 050018
  • 出版日期:2014-06-25 发布日期:2014-07-03
  • 通讯作者: 张 岩 E-mail:snowwinglv@126.com
  • 基金资助:

    国家质检总局科技计划项目(2010QK109)

Determination of Characteristic Proteins in Plant Protein Foods by High Performance Capillary Electrophoresis

LI Ke-hong1, BAI Yan-kun1, ZHOU Wei1, ZHANG Yan1,*, PANG Jun2, GAO Wen-hui2   

  1. 1. Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050091, China;
    2. College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
  • Online:2014-06-25 Published:2014-07-03
  • Contact: ZHANG Yan E-mail:snowwinglv@126.com

摘要:

植物蛋白广泛应用于食品加工中,相关食品标准迫切需要建立蛋白质定性、定量的检测方法保证食品质量和安全。采用高效毛细管电泳法,对4 种样品中的蛋白质进行检测,并利用指纹图谱软件对样品中所含蛋白种类进行比对,找出同类样品中共有特征峰,以及对不同植物蛋白互相掺假谱图进行比对,通过特征峰及保留时间对掺假物质进行鉴别,为植物蛋白掺假鉴别实验提供科学依据。

关键词: 高效毛细管电泳, 检测, 植物蛋白, 特征蛋白质

Abstract:

As plant proteins are widely used in food processing, analytical methods for the qualification and quantification of
these proteins are urgently needed for food standards to ensure food quality and safety. In this experiment, protein contents
were determined in four samples using capillary electrophoresis and protein identification was performed by comparison with
standard fingerprints to find out common characteristic peaks. Moreover, mixed plant proteins were chromatographed and
identified by comparison of characteristic peaks and retention times. This study may lay the foundation for the identification
of plant protein adulteration.

Key words: high performance capillary electrophoresis, detection, plant protein, characteristic protein

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