关键词: 甲醛, 鸭血制品, 高效液相色谱法

Abstract:

A high performance liquid chromatography (HPLC) method for the determination of formaldehyde in duck blood
products is presented in this paper. The formaldehyde in sample was extracted and derivatized with a mixture of 2,4-dinitrophenylhydazine
dissolved in acetonitrile (300 mg/500 mL) and water (1:1, by volume), and separated on a Waters SunFire
C18 column (4.6 mm × 250 mm, 5 μm), using a mobile phase composed of methanol and water (65:35, by volume) at a
flow rate of 1.0 mL/min. The compound was detected by UV detector at 365 nm and then quantified by external calibration
method. The results showed that the proposed method displayed good linear relationship over the concentration range of
0.4–6.0 μg/mL, with a correlation coefficient of 0.999 7. The limit of detection (LOD) was 0.2 mg/kg, and the precision
expressed as relative standard deviation (RSD) for 6 replication determinations was blew 5%. The average recoveries
for blank spiked samples were in the range of 85.1%–92.7% (n = 3). This method is simple and convenient with good
separation for the analysis of formaldehyde in duck blood products.

Key words: formaldehyde, duck blood products, high performance liquid chromatography (HPLC)